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E u r o S c i C o n C o n f e r e n c e o n
Chemistry
2018
Chemistry 2018
Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
F e b r u a r y 1 9 - 2 0 , 2 0 1 8
P a r i s , F r a n c e
Page 66
P
articulate matter (PM) such as ultrafine particulate matter
(UFP) and the organic compound pollutants such as
polycyclic aromatic hydrocarbon (PAH) are widespread in the
environment. UFP and PAH are present in the air, and their
presence may enhance their individual adverse effects on
human health. However, the mechanism and effect of their
combined interactionsonhumancells arenotwell understood.
We investigated the combined toxicity of silica nanoparticles
(SiNPs) (UFP) and Benzo[a]pyrene (B[a]P) (PAH) on human
endothelial cells. Human umbilical vascular endothelial cells
(HUVECs) were exposed to SiNPs or B[a]P, or a combination
Combined effect of
silica nanoparticles
and benzo[a]pyrene
on cell cycle
arrest induction
and apoptosis in
human umbilical vein
endothelial cells
Collins Otieno Asweto
1, 2
, Jing
Wu
1
, Hejing Hu
1
, Lin Feng
1
,
Xiaozhe Yang
1
, Junchao
Duan
1
and Zhiwei Sun
1
1
Capital Medical University of Beijing, China
2
University of Embu, Kenya
of SiNPs and B[a]P. The toxicity was investigated by assessing
cellular oxidative stress, DNA damage, cell cycle arrest, and
apoptosis. Our results show that SiNPs were able to induce
reactive oxygen species generation (ROS). B[a]P, when acting
alone, had no toxicity effect. However, a co-exposure of SiNPs
and B[a]P synergistically induced DNA damage, oxidative stress,
cell cycle arrest at the G2/M check point, and apoptosis. The co-
exposure induced G2/M arrest through the upregulation of Chk1
and downregulation of Cdc25C, cyclin B1. The co-exposure also
upregulated bax, caspase-3, and caspase-9, the proapoptic
proteins, while down-regulating bcl-2, which is an antiapoptotic
protein. These results show that interactions between SiNPs
and B[a]P synergistically potentiated toxicological effects on
HUVECs. This information should help further our understanding
of the combined toxicity of PAH and UFP.
asweto_collins@yahoo.comJ Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C1-003