20 / 32
E u r o S c i C o n C o n f e r e n c e o n
Chemistry
2018
Chemistry 2018
Journal of Organic & Inorganic Chemistry
ISSN 2472-1123
F e b r u a r y 1 9 - 2 0 , 2 0 1 8
P a r i s , F r a n c e
Page 65
S
epticemia is a serious disease that requires early
diagnosis, and procalcitonin (PCT) serves as a diagnostic
biomarker for this disease. Therefore, a rapid, sensitive, and
cost-efficient platform is urgently needed for septicemia
detection.Thefiber opticparticleplasmon resonance (FOPPR)
biosensor based on the integration of gold nanoparticles and
fiber optic evanescent wave may provide an ultrasensitive and
rapid detection approach. Herein, an ultrasensitive sandwich
strategy in the FOPPR sensing platform for PCT detection has
been developed. The results demonstrate that the proposed
sandwich strategy for PCT provides a wide linear response
Using sandwich
method to increase
the detection
sensitivity of
procalcitonin (PCT) in
fiber optic particle
plasmon resonance
biosensor
Chang-Yue Chiang
1
, Ting-
Chou Chang
1
, Chia-Wei Hsu
1
,
Chun-Jen Huang
2
, Tze-Ta
Huang
1
and Lai-Kwan Chau
1
1National Chung Cheng University, Taiwan
2National Central University, Taiwan
range from 1 pg/mL to 100 ng/mL and an extremely low limit
of detection (LOD) of 0.28 pg/mL (0.021 pM). In addition, the
sandwich strategy has advantages of convenience, low-cost,
short analysis time (15 min), good specificity, acceptable
stability and reproducibility. Finally, the results from the FOPPR
biosensor using the sandwich strategy were compared to a
clinically validated electrochemiluninescence assay (Roche
cobas e411). An excellent correlation coefficient (r=0.99)
between the two methods for blood plasma samples from 12
septicemia patients was achieved, indicating that the FOPPR
biosensor using the new sandwich strategy has the potential
to be used in actual clinical diagnosis of PCT. Furthermore, it
is also potentially applicable to the detection of many other
biomarkers, and thus provides an ideal technical tool for point-
of-care diagnostics.
standford_chiang@hotmail.comJ Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C1-003