brain-injury-2017-keynote

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Neurosurg, an open access journal
ISSN: 2471-9633
Page 16
Notes:
August 14-16, 2017 | Toronto, Canada
BRAIN DISORDERS AND DEMENTIA CARE
4th International Conference on
alliedacademies
Statement of the Problem: Demyelination that is the
hallmark of multiple sclerosis is a complex process that partly
involves deimination of myelin basic protein. Peptidylarginine
deiminase (PAD) family of enzymes catalyze the deimination
of protein-bound arginine to citrulline. The 2 isoform of
PAD is present in the myelin sheet of both the CNS and the
PNS. Resveratrol, a polyphenol with neuroprotective effects
unexpectedly has shown to exacerbate demyelination in
models of multiple sclerosis. The purpose of this study
was to evaluate the effects of resveratrol on the enzymatic
activity of PAD and on the mRNA levels of the 2 isoform of
PAD in the brain cortex of rats. Methodology & Theoretical
Orientation: Rats were gavaged with resveratrol or water
(control) and or ethanol (vehicle) for 3 weeks. Cortical tissues
were assayed for the PAD activity. Real-time RT-PCR was used
to assess the mRNA levels of PAD2. Findings: Resveratrol
at the dose of 120mg/Kg bw/day significantly increased
(P<0.05, Tukey’s test) the activity of PAD2 in the brain cortex
of rats as compared to the control group. Levels of the mRNA
for PAD2 in the cortex of rats treated by resveratrol was
not significantly different (P>0.05 Tukey’s test) from those
treated by water or ethanol. Conclusion & Significance: The
finding that oral resveratrol induces PAD activity in the cortex
of rats is in line with the adverse effects of resveratrol on
multiple sclerosis.
Speaker Biography
Ali Akbar Owji is an Emeritus Professor in the Department of Biochemistry at the Shiraz
University of Medical Sciences, Iran. He received his BSc in Chemistry and his PhD in
Medical Biochemistry from Tehran University, Iran. His research has mainly concerned
the Pharmacological and functional characterization of receptors for neuropeptides in
the CNS. He has also worked on the expression pattern of various BDNF transcripts and
on the epigenetic changes around the promoters of BDNF gene in the rat brain during
tolerance to and withdrawal from morphine. During the past eight years, he has also
worked on the expression status of the novel human genes UBE2Q2 and UBE2Q1 in hu-
man tumors and cell Lines. His career has also involved teaching Medical Biochemistry,
Molecular Endocrinology and Biochemistry of the Nervous System. He has supervised
six Ph.D and fifteen MSc students to completion.
e:
Ali Akbar owji
SUMAS, Iran
Effects of Resveratrol on the activity and transcript levels of peptidyl arginine
deiminase in the brain cortex of rats
Ali Akbar owji, Neurosurg 2017, 2:2
DOI: 10.21767/2471-9633-C1-004