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Volume 3, Issue 2
Insights in Analytical Electrochemistry
ISSN: 2470-9867
Analytical Chemistry-Formulation 2017
August 28-30, 2017
Page 56
8
th
Annual Congress on
&
14
th
International Conference and Exhibition on
August 28-30, 2017 Brussels, Belgium
Analytical and Bioanalytical Techniques
Pharmaceutical Formulations
Simultaneous sensing of ascorbic acid, dopamine, uric acid and L-tryptophan usingAgNPs, graphene
oxide and poly (L-Arginine) composite
Gözde Aydogdu Tıg
Ankara University, Turkey
E
lectro-analytical methods are routinely used in analytical applications and clinical investigations to determine the
concentration of biological compounds in human body fluids. Ascorbic acid (AA), dopamine (DA), uric acid (UA) and
L-tryptophan (L-Trp) play critical roles in human metabolism, central nervous and renal systems. Abnormal concentration
levels of these biomolecules can cause serious health problems such as mental illness, cancer, Parkinson’s disease, hyperureaemia
and gout. It is known that AA, DA, UA and Trp usually co-exist in biological samples such as serum and urine. Therefore, it is
highly desirable to develop a sensitive method that can determine these biomolecules simultaneously in diagnostic research and
analytical applications. However, the oxidation potentials of these compounds are too close to be separated at bare electrodes
owing to their overlapping signals. Recently, metal/metal oxides nanoparticles have received great attention as a modifier
material in developing electrochemical sensors due to their excellent properties such as high electron transfer ability, easy
synthesis and small size. Graphene oxide (GO) which has a layered structure with a large specific area has been extensively used
in the recent years for bio-sensing applications. Nowadays, graphene/conducting polymers composites have been considered
as one of the most promising functional components due to their good electrical conductivity, chemical stability and high
electrochemical capacity. Electropolymerization of amino acids have gained great attention in the field of sensors/biosensors.
Among the poly (amino acids), poly (L–arginine) (P (Arg)) has attracted great attention in constructing biosensors owing to
its functional –NH
2
and –COOH groups and it could electrostatically interact with negatively charged groups of GO. In this
study, a composite glassy carbon electrode (GCE) based on AgNPs, GO and P (Arg) was prepared for the determination of AA,
DA, UA and L-Trp. The composite was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy
(EIS) and scanning electron microscopy (SEM). All compounds can be well separated by their different peak potentials and
they can be simultaneously detected in the quaternary mixture. Moreover, this proposed sensing strategy revealed excellent
stability and reproducibility. The potential application of the modified electrode for sensing these compounds in human urine
samples was also investigated.
gaydogdu@science.ankara.edu.trGözde Aydogdu, Insights in Analytical Electrochemistry, 3:2
DOI: 10.21767/2470-9867-C1-002