advanced-stem-cell-2018-scientifictracks

3 / 12

Advanced Stem Cell 2018 Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725 Page 21
December 03-04, 2018
Valencia, Spain
15th Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
Cell-based therapy is not a new concept; it is considered
as one of the most promising approaches for treating
diseases and for regenerative medicine. In addition, effective
cell therapy can greatly benefit from the ability to monitor
transplanted stem cells post-intervention. Mesenchymal stem
cells (MSCs) represent one of the leading candidate population
for regenerative medicine. Superparamagnetic iron oxide
nanoparticles (SPIONs) represent contrast agents offering a
possible way to track labelled cells after administration using
MRI. Moreover, we have demonstrated that these magnetic
particles (MPs) do not affect cell viability, proliferation,
differentiation or migration. The aim of the present study is to
determine the ability to use these iron particles to label MSCs
and test their potential to control cell migration when exposed
to a magnet. This aim was achieved by culturing labelled and
unlabelled cells in 2D and 3D models in presence or absence
of magnet. Significant response to magnet exposure was
observed in 2D culture where 76% of labelled cells moved to
the magnet side when compared to unlabelled cells. There was
only 45% of unlabelled cells found to have moved to the magnet
side. Additionally, 64% of labelled cells moved to the magnet
side in a 3D culture model, while the unlabelled cells showed
around 50% cells moving to the magnet side. Briefly, we have
shown that MSCs can be labelled with MPs in vitro, and this
strategy can contribute in improving the spatial tracking of
transplanted stem cell and therefore improve their efficiency
for therapeutic applications.
Biography
Reem Alkharji is a Senior Medical Laboratory Specialist; has completed her
Bachelor’s Degree in Clinical Laboratory Sciences. She supported her labo-
ratory work experiences in the hospital with two masters degree; Master’s
in Health and Hospital Administration andMaster’s in StemCell Technology.
Stem cell research is her passion to improve patients’ life. She believes that
some waste in the medical field (as umbilical cord blood) could be changed
to worth products and the quality of people life improve if provided the right
service at the right time.
Mesenchymal stem cell labelling using
magnetic particles for in vitro applications
Reem Alkharji1,2 and Sottile V2
1Prince Sultan Military Medical City, KSA
2University of Nottingham, UK
Reem Alkharji et al., J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-002