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Journal of Stem Cell Biology and Transplantation
ISSN: 2575-7725
December 03-04, 2018
Valencia, Spain
Advanced Stem Cell 2018
Page 15
15
th
Edition of EuroSciCon Conference on
Advanced Stem Cell &
Regenerative Medicine
T
itanium implants surfaces are continuously modified to
improve biocompatibility and to promote osteointegration.
Graphene oxide (GO) has been successfully used to ameliorate
biomaterial performances in terms of implant integration with
host tissue. The aim of this study is to evaluate dental pulp stem
cells (DPSCs) cell viability, cytotoxic response and osteogenic
differentiation capability in the presence of GO-coated titanium
surfaces. Experimental discsweredivided into: standard titanium
(control), titanium treated with inorganic ions (test), both coated
with GO (ctrl+GO and test+GO, respectively). Surface analysis
was realized by atomic force microscopy (AFM), morphological
analysisbyscansionmicroscopy (SEM), proliferation ratebyMTT,
cytotoxic response by LDH assay, osteoblastic differentiating
potential by real-timeRT-PCRof BMP2, RUNX2, SP7andCollagen
I, and by measuring the secretion of PGE2. SEM analysis shows
extracellular matrix deposition in all samples, in test and test+GO
more evident after 14 days of culture. MTT analysis shows,
after 7 and 14 days of culture, a significant viability increase on
test+GO sample; LDH assay reveals no cytotoxic response in all
the experimental points. An increase of osteogenic markers, and
of PGE2 secretion level at later stages, is recorded on test+GO.
The obtained results evidence that the tested biomaterials
stimulate cell viability and that they are not cytotoxic. However,
GO enrichment of the test surface is also capable to better
and faster induce osteogenic differentiation, thus suggesting
this biomaterial as a new promising surface to promote bone
remodeling process improving dental implants integration with
host tissue.
Biography
Susi Zara pursued her graduation (2006) in Pharmacy; PhD in 2009 from
the University of G d Annunzio of Chieti Pescara, Italy. She is now a per-
manent researcher in the Pharmacy Department at the same university.
She has published more than 50 full length papers in international peer-re-
viewed journals. Her fields of research are represented by intracellular
signaling driving differentiation of dental pulp mesenchymal stem cells in
the presence of innovative biomaterials with a potential use in dental and
orthopedic regenerative medicine.
susi.zara@unich.itDental pulp stem cells osteblastic
differentiation on graphene oxide-
coated titanium surfaces: an in
vitro study
Susi Zara
University of G d Annunzio Chieti Pescara Italy
Susi Zara, J Stem Cell Biol Transplant 2018, Volume 2
DOI: 10.21767/2575-7725-C1-001
Roberta Di Carlo
1
, Antonella Fontana
1
, Alessia Ventrella
1
, Barbara Zavan
2
, Gabriella
Teti
3
, Mirella Falconi
3
and
Amelia Cataldi
1
1
University of G d Annunzio Chieti Pescara, Chieti, Italy
2
University of Padova, Italy
3
University of Bologna, Italy