advanced-stem-cell-2018-keynote

Journal of Stem Cell Biology and Transplantation

ISSN: 2575-7725

December 03-04, 2018

Valencia, Spain

Advanced Stem Cell 2018

Page 14

Edition of EuroSciCon Conference on

Advanced Stem Cell &

Regenerative Medicine

esenchymal stem cells (MSCs) are widely used in clinical

trials for various conditions. We have developed easy

methods for the isolation of MSCs from bone marrow, adipose

tissue, umbilical cord and developed an in-house platelet lysate

processing method for xeno free propagation of MSCs from the

three sources. Further, we developed cryopreservation methods

and cGMP compliant system for the procurement of MSCs

for various clinical trials, including for treatment of large bone

defect, nonunion fracture, spinal cord injury, near blindglaucoma,

osteoarthritis of the knee and third degree burn. Our large scale

good quality MSC production protocol is called Universitas

Indonesia-Cipto Mangunkusumo (UI-CM) method. The aim of

this presentation is to summarize themain findings in large scale

good quality production of MSCs from the three sources in terms

of the yield, their surface marker characteristics, differentiation

capacities, problems in obtaining good quality, unmatched

between cell readiness and demand in clinical trials and finally

tips and tricks to overcome the problems.

Biography

Jeanne Adiwinata Pawitan pursued her graduation asMedical Doctor from

the Faculty of Medicine at Universitas Indonesia (FMUI) and Master’s De-

gree inMedical Biology and started working in the Department of Histology

at FMUI. She also pursued her PhD from Osaka University, Osaka, Japan

and continued working in the Department of Histology, FMUI and was ap-

pointed as Professor in 2005. Currently, she isworking at StemCell Medical

Technology Integrated Service Unit, RSCM-FMUI as GLP Lab Coordinator

and at StemCell and Tissue Engineering Research Center, Indonesia Medi-

cal Education and Research Institute (IMERI), FMUI as Lab Manager.

Large scale good quality

mesenchymal stem cell

production: Indonesian

experience

Jeanne Adiwinata Pawitan

University of Indonesia, Indonesia

Jeanne Adiwinata Pawitan, J Stem Cell Biol Transplant 2018, Volume 2

DOI: 10.21767/2575-7725-C1-001