advanced-nano-2017-posters-accepted-abstracts

15 / 31

Nano Research & Applications
ISSN 2471-9838 Advanced Nano 2017
Page 67
September 11-12, 2017 Amsterdam, Netherlands
20th International Conference on
Advanced Nanotechnology
Mesenchymal stem cells differentiation into
cardiac linage on modified nanofiber scaffold
Arun Kumar1, 2 and Edward Marks2
1Thomas Jefferson University, Philadelphia
2University of Delaware, USA
The cardiovascular disease and death has led
researchers to look beyond pharmaceutical standards
ofcaretonewcell-basedtherapiesthatmaybenefitpatients.
Annually in the United States alone 600,000 people die
of heart disease. The innovation is to use the modified
nanofiber scaffold and (hBMSC) human bone marrow
derived stem cells as a regenerative medicine strategy
to replace damaged cardiac tissue after traumatic events
such as a heart attack . The induced pluripotent stem cells
(iPSCs) allows the development of beating cardiac tissue
in vitro. Recent research has demonstrated two advances
in hBMSC therapy for cardiac healing: first, priming
the cells in vitro for eventual transplantation aides cell
survival and terminal differentiation once deposited to the
cardiac niche and second, paracrine factors produced by
hBMSCs while differentiating in culture. These discoveries
lead us to develop the modified nanofiber scaffold based
hBMSc to differentiate in to cardio myocytes to repair the
damaged heart. Cell-based therapies as treatment for
MI (Myocardial Infraction) have demonstrated safety in
vivo but mixed efficacy. We developed an adjustable rat
model of MI (Myocardial Infraction) to test the therapeutic
effectiveness of intracardiac injections of hBMSCs primed
on protein- and small molecule-coated nanoscaffolds
which has demonstrated increased cardiac biomarker
expression and decreased canonical WNT signaling.
Myocardial infraction (MI) rat model to mimic the human
MI conditions is created by subcutaneous administration
of isoproterenol resulted in dose dependent myocardial
damage. After intracardiac injections, hBMSCs combined
with nanofiber scaffold engrafted within the heart and
provided increased EF in animals given low and medium/
high cardiac damage, compared to sham operated rats.
These results demonstrate stem cells based therapies
are not conducive to all levels of MI severity, and future
stem cell trials will be aided by standardizing definitions of
cardiac improvement.
Nano Research & Applications