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May 24-25, 2018
London, UK
Vascular Surgery 2018
3
rd
Edition of World Congress & Exhibition on
Vascular Surgery
Journal of Vascular and Endovascular Therapy
ISSN: 2573-4482
Statement of the Problem:
Toll-like receptors (TLRs) are a
family of highly conserved, transmembrane proteins that
recognize a wide spectrum of pathogen-associated molecular
patterns (PAMPs) and activate innate immune response.
It was recently shown that TLR2, TLR3, and TLR4 signaling
contributes to abdominal aortic aneurysm (AAA) formation
and development. However, there are no studies regarding the
association between single nucleotide polymorphisms within
TLR genes and the incidence of AAAs. The aim of this study
was to determine the prevalence of TLR2, TLR3, and TLR4
polymorphisms, and investigate the relationship between these
polymorphisms and AAA risk.
Methodology & Theoretical Orientation:
The presence of the
seven TLR SNPs was studied using polymerase chain reaction-
restriction fragment length polymorphism (PCR-RFLP), whereas
a selected cytokine levels were quantified by enzyme-linked
immunosorbent assays (ELISAs).
Findings:
The heterozygous genotype of the SNP rs121917864
of TLR2 was detected more frequently in patients with AAA and
was associated with an almost six-fold increased risk of AAA
(P<0.001) and larger aneurysm size (P=0.037). In contrast, a
relationship between the heterozygous CA genotype of TLR3
SNP rs3775296 and diminished risk of AAA formation and
development was found (P<0.001).
Conclusion & Significance:
The heterozygous genotype of TLR2
SNP rs121917864 may be a predictor of AAA formation, while
TLR3 SNP rs3775296 seems to exert an independent protective
effect. It is therefore essential to identify the host determinants
of aneurysm formation in humans and understanding the role of
the selected TLRs SNPs in the pathogenesis of AAA.
Recent Publications
1. Lai C H, Wang K C, Lee F T, Tsai H W, Ma C Y, Cheng
T L, Chang B I, Yang Y J, Shi G Y and Wu H L (2016)
Toll-like receptor 4 is essential in the development of
abdominal aortic aneurysm. PLoS One 11:e0146565.
2. Vorkapic E, Lyrundberg A M, Mäyränpää M I,
Eriksson P and Wågsäter D (2015) TRIF adaptor
signaling is important in abdominal aortic aneurysm
formation. Atherosclerosis 241:561-568.
3. Yan H, Cui B, Zhang X, Fu X, Yan J, Wang X, Lv X, Chen
Z and Hu Z (2015) Antagonism of toll-like receptor
2 attenuates the formation and progression of
abdominal aortic aneurysm. Acta Pharm Sin B 5:176-
187.
4. Aoyama N, Suzuki J, Ogawa M, Watanabe R,
Kobayashi N, Hanatani T, Ashigaki N, Sekinishu A,
Izumi Y and Isobe M (2013) Toll-like receptor-2 plays
a fundamental role in periodontal bacteria-accelerated
abdominal aortic aneurysms. Circ J 77:1565-1573.
5. Koch A, Pernow M, Barthuber C, Mersmann J,
Zacharowski K and Grotemeyer D (2012) Systemic
inflammation after aortic cross clamping is influenced
by Toll-like receptor 2 preconditioning and deficiency.
J Surg Res 178:833-841.
Insight into single nucleotide polymorphisms of Toll-like
receptors and the risk of abdominal aortic aneurysm formation
Agnieszka Jabłońska
Polish Academy of Sciences, Poland
Agnieszka Jabłońska, J Vasc Endovasc Therapy 2018, Volume 3
DOI: 10.21767/2573-4482-C1-002
Figure 1:
Schematic representation of the
different SNPs in TLR genes.