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Journal of Clinical Immunology and Allergy
ISSN: 2471-304X
E u r o p e a n C o n g r e s s o n
Vaccines & Vaccination
and Gynecologic Oncology
Vaccines & Vaccination and Gynecologic Oncology 2018
O c t o b e r 2 6 - 2 7 , 2 0 1 8
B u d a p e s t , H u n g a r y
H
emorrhagic Septicemia (HS) is a fatal disease of bovines caused by
Pasteurella multocida
. Marker vaccine could help in
control of HS. Bovine brucellosis is an important zoonotic disease causing huge economic losses worldwide. Currently
no effective therapy is available for Brucellosis and animals remain carrier lifelong. We have developed novel marker vaccine
for HS and therapeutic vaccine for brucellosis employing bacteriophages.
P. multocida
(B: 2) has grown under iron restricted
conditions followed by lysis with a lytic
Pasteurella phage
was used as the marker vaccine. Cattle in phage lysate vaccine (PLV)
group showed higher antibody titers compared to alum precipitated vaccine (APV) group as revealed by ELISA. Mice and rabbits
vaccinated with PLV revealed significantly higher antibody titres than mice and rabbits receiving APV by ELISA (P<0.001). The
peak log10 values (3.46) in case of PLV mice by ELISA were attained at 90 days post inoculation (DPI) whereas in APV mice, the
peak value at 90 DPI was 2.82. Mean log10 titres by ELISA in PLV and APV rabbits were 2.43 and 2.35, respectively at 30 DPI
whereas at 120 DPI, the titres were 3.29 and 2.75, respectively. The marker vaccine induced higher and longer immune response
in cattle, mice and rabbits compared to the APV. We have developed a therapeutic vaccine for bovine brucellosis employing phage
lysates of RB51 (RL) and S19 (SL) strains of
Brucella abortus
. The SL induced strong antibody response and RL stimulated cell
mediated immunity. In vitro restimulation of leukocytes from RL immunized cattle induced interferon gamma production. A single
subcutaneous dose of 2 ml of cocktail lysate (both RL and SL), eliminated live virulent
Brucella
from Brucellosis affected cattle.
The plasma level of
Brucella
specific 223 bp amplicon became undetectable by RTPCR and blood was negative for live
Brucella
in 3 months post-immunization as evident by culture.
drhmsaxena@gmail.comPhage lysed bacteria as vaccines against
Pasteurella and
Brucella
infections in cattle
Hari Mohan Saxena, Sabia Qureshi and Sugandha Raj
Guru Angad Dev Veterinary and Animal Sciences University, India
Journal of Clinical Immunology and Allergy, Volume: 4
DOI: 10.21767/2471-304X-C2-006
Euro Vaccines 2018