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Plant Genomics 2019
June 13-14, 2019
Berlin, Germany
Asian Journal of Plant Science & Research
ISSN: 2249-7412 Page 22
Plant Genomics
5th Edition of International Conference on
Virulence of Fusarium circinatum is associated with
perturbation of phytohormone homeostasis in Pinus
pinaster seedlings
Laura Hernandez Escribano
National Institute of Agricultural and Food Research and Technology, Spain
Plants have developed complex molecular strategies
to face the attack of a pathogen in order to maintain
their survival, and phytohormones are known to play a
crucial role in plant-pathogen interactions. The aim of
this study is to elucidate the role of phytohormones in
Fusarium circinatum virulence, the causal agent of pitch
canker disease, known as one of the most important
pathogens of conifers worldwide.
Methodology and Theoretical Orientation: For this
purpose, by a dual RNA-sequencing approach, we
determine the expression profiling of both organisms
during the interactionat 3, 5and10days post-inoculation.
Findings: Pinus pinaster showed moderate resistance
at the early time points. This may be explained, at
least in part, by the early recognition, the induction of
pathogenesis-related proteins and the activation of
complex phytohormone signaling that involves crosstalk
between threemain protagonists: Salicylic acid, jasmonic
acid and ethylene. Moreover, we hypothesise the key
steps where the pathogen could be manipulating host
phytohormone balance to its own benefit, contributing to
pathogen virulence. Upon examination of the pathogen
transcripts, we propose that F. circinatum prevents
salicylic acid biosynthesis from the chorismate pathway
by the synthesis of isochorismatase family hydrolase
(ICSH) genes, perturbs ethylene homeostasis in the host
by expression of genes related to ethylene biosynthesis,
and could be blocking jasmonic acid signalling by COI1
suppression.
Conclusion and Significance: Targeted functional testing
using F. circinatum mutants in future studies would be
needed to support this hypothesis.
Biography
Laura Hernández Escribano is currently a PhD student in the
National Institute of Agricultural and Food Research and Tech-
nology, Center for International Forestry Research (INIA-CI-
FOR), working in the field of plant pathology with the thesis
named “Fusarium circinatum – host interaction: Ecological
and molecular aspects of the pathogenic and endophytic as-
sociation”. She has a degree in Biology andmasters in “Applied
Vegetable Biology”, by the Complutense University of Madrid.
Laura Hernandez Escribano, AJPSKY 2019, Volume 09