pharma-2018-keynote

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E u r o p e a n C o n g r e s s o n
Pharma
American Journal of Pharmacology and Pharmacotherapeutics
ISSN: 2393-8862
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Pharma 2018
Page 18
In this study, a sensitive high-performance liquid chromatography with tandem
mass spectrometry (HPLC-MS/MS) method established for simultaneously
determining five main isosteroidal alkaloids (imperialine-3-β-D glucoside
(imperialine-G), imperialine, peimine, hupehenine and yibeinoside A) in Siberian
Fritillary Bulb, was applied to pharmacokinetic studies in rat plasma. The
plasma samples pretreated using liquid-liquid extraction with ethyl acetate were
quantitated by multiple reaction monitoring (MRM) via positive electrospray
ionization (ESI) mode. Chromatographic separation was performed on an Intersil
ODS-2 column (5μm, 4.6 ×150mm) with a single fifteenminutes run using gradient
elution. Themobile phrase consisted of (A) 10mMammoniumacetate (containing
0.1% of formic acid) and (B) methanol. Method validation results showed that
the developed method had good accuracy and precision over the corresponding
linearity range for all the analytes. Besides, bench-top, autosampler, freeze-thaw
circulation and long-term storage stabilities met the acceptable limit. This study
examined a specific and robust method which was successfully applied to analyze
rat plasma samples for pharmacokinetic study of five isosteroidal alkalosids.
Biography
Liming Ye has obtained his PhD Degree in 2008 from West
China School of Pharmacy, Sichuan University, Chengdu,
Sichuan Province, RP China. He is Professor of Pharmaceutical
Analysis in West China School of Pharmacy, Sichuan University.
His research interest is in the area of basic components
analysis, the dynamic change rule study and standardization
research of Traditional Chinese Medicine (TCM), as well as the
establishment and study of predictive quantitative retention-
activityrelationshipmodelsofseriesmedicinebybiopartitioning
micellar chromatography (BMC).
Simultaneous determination of five
isosteroidal alkaloids in Siberian Fritillary
Bulb in rat plasma and its application in
pharmacokinetic study by HPLC-MS/MS
Liming Ye, Min Liang, Jianzhong Wang and
Yanping Liu
Liming Ye et al., Am J Pharmacol Pharmacother 2018, Volume 5
DOI: 10.21767/2393-8862-C1-001