occupational-health-2018-posters-abstracts

Occupational Health 2018

Journal of Nursing and Health Studies

ISSN: 2574-2825

Page 71

May 28-29, 2018

London, UK

Edition of International Conference on

Occupational Health and

Safety

Exposure to Cadmium or Lead is generally considered as an

occupational health hazard while; contaminated ground water

is the major route for human exposure to arsenic. An early and

chronic exposure of these heavy metals may result in reduced or

impaired fertility at later stages of life. This study aims to assess

and compare the toxic effects of Sodium Arsenite, Cadmium

Chloride and Lead Acetate in both reproductive (testes) and non-

reproductive tissues (liver) of juvenileandadult rats.Triplicatesets

ofmale juvenile and adult Wistar ratswere supplied drinkingwater

having heavy metal salts of dose 100 times higher thanMaximum

Contamination Limit, for three months and simultaneously, age-

matched controls were taken. Effects of heavy metals were

assessed by studying total sperm count and defective sperm and

by histological examinations. Semi-quantitative RT-PCRwas done

in order to assess the expression level of stress proteins mRNAs

i.e. Heat Shock Protein 70 (HSP70) and Metallothioneins (MT1

and MT2) in testes and liver of all treated and control rats. Results

showed that heavy metal exposure (except arsenic) caused a

significant decrease in healthy sperm count (p<0.05) and tissue

integrity. The expression of HSP70 and MT1 were found higher

in treated tissues (significantly in juveniles) compared to controls

(p<0.05). Furthermore, higher expression of MT1 mRNA can

make this gene a good biomarker to assess heavy metal toxicity.

This study showed that juveniles are showingmore severe effects

compared to adults justifying the vulnerability of early chronic

exposure to heavy metals.

Chronic exposure to heavy metals declines sperm quality,

damages tissue architecture and alters expression of stress

proteins

Sujata De Chaudhuri

and

Dyutiman Mukhopadhyay

Barrackpore Rastraguru Surendranath College, India

University of Calcutta, India

J Nurs Health Stud 2018, Volume 3

DOI: 10.21767/2574-2825-C2-006