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Volume 3, Issue 4 (Suppl)
Nano Res Appl
ISSN: 2471-9838
November 07-08, 2017 Singapore
International Meeting on
Advanced Nanomaterials and Nanotechnology
Nanomaterials Meetings 2017
November 07-08, 2017
Page 29
pH-responsive Doxorubicin-loaded cockle shell-derived nanoparticles:
Release kinetics and pharmacokinetics in canine
Danmaigoro Abubakar, Gayathri, Thevi Selvarajah, Moh’d, Hezmee, Moh’d Noor, Rozi Mahmud and Md Zuki Abu Bakar
Universiti Putra Malaysia, Malaysia
N
anoparticles with stimuli-responsive release mechanism have received great interest in nanomedicine. Doxorubicin-loaded pH-
responsive nanocarriers could enable selectivity and specificity by reducing premature drug release in the plasma following
an intravenous administration. Doxorubicin-load cockleshell-derived nanoparticle (CSNP-DOX) was prepared via ball-milling
method. Apart from the analysis of CSNP drug release kinetics at pH 4.8 and 7.4, a high-performance liquid chromatography (HPLC)
bioanalytical method was developed for the detection of doxorubicin. For the pharmacokinetics of CSNP-DOX, Animal ethics
approval was sought. Six canines were divided into two groups to receive intravenous CSNP-DOX and free doxorubicin at 30 mg/
m
2
, respectively. At pre-determined time interval, blood was sampled and processed before analyzed by HPLC. The pharmacokinetic
parameters were determined based on the plasma doxorubicin concentration in the canines. An excellent bioanalytical method with
high acceptable extraction yield and linearity of 89.87% and 0.997 within the range limit of 0.25-4 µg/mL was revealed from the
method developed. At pH 7.4, 13.7% of DOX was released from CSNP-DOX after 96 hours while 52.6% of doxorubicin was recorded
in the free doxorubicin alone. However, the amount of doxorubicin released from the nanocarriers doubled in acidic condition.
CSNP-DOX increases the t
1/2
, T
max
and AUC
0-t
of doxorubicin. The plasma concentration of doxorubicin rapidly becomes lower versus
time when compared to the plasma concentration of CSNP-DOX. CSNP-DOX exhibited pH-triggered and sustained-drug release
properties. The pharmacokinetic parameters confirmed that CSNP has the ability to regulate and delay the release of doxorubicin in
blood circulation.
Biography
Danmaigoro Abubkar is currently a PhD student at Universiti Putra Malaysia. He is also a Lecturer at the Usmanu Danfodiyo
University, Nigeria at the Department of Veterinary Anatomy.
abubakar.danmaigoro@udusok.edu.ngDanmaigoro Abubakar et al., Nano Res Appl 2017, 3:4
DOI: 10.21767/2471-9838-C1-005