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NanoMat 2018
Nano Research & Applications
ISSN: 2471-9838
Page 91
April 26-27, 2018
Rome, Italy
17
th
Edition of International Conference on
Emerging Trends in
Materials Science and
Nanotechnology
C
artilage is a dense connective tissue with limited self-repair
properties. Currently, the therapeutic use of autologous or
allogeneic chondrocytes makes up an alternative therapy to the
pharmacologicaltreatment.Thedesignofabioprinted3Dcartilage
with chondrocytes and biodegradable biomaterials offers a new
therapeutic alternative able of bridging the limitations of current
therapies in the field. We have developed an enhanced printing
processes-injection volume filling (IVF) to increase the viability
and survival of the cells when working with high temperature
thermoplastics without the limitation of the scaffold geometry
in contact with cells. We have demonstrated the viability of the
printing process using chondrocytes for cartilage regeneration.
An alginate-based hydrogel combined with human chondrocytes
(isolated fromosteoarthritis patients) was formulated as bioink-A
and the polylactic acid as bioink-B. The bioprinting process
was carried out with the REGEMAT V1 bioprinter (Regemat 3D,
Granada-Spain) through an IVF. The printing capacity of the
bioprinting plus the viability and cell proliferation of bioprinted
chondrocytes was evaluated after five weeks by confocal
microscopy and alamarBlue assay (Biorad). Results showed
that the IVF process does not decrease the cell viability of the
chondrocytes during the printing process as the cells do not
have contact with the thermoplastic at elevated temperatures.
The viability and cellular proliferation of the bioprinted artificial
3D cartilage increased after five weeks. In conclusion, this study
demonstrates the potential use of Regemat V1 for 3D bioprinting
of cartilage and the viability of bioprinted chondrocytes in the
scaffolds for application in regenerative medicine.
bd1@regemat3d.comDesign of 3D bioprinted scaffolds for cartilage regeneration
Gloria Pinilla Garcia
REGEMAT 3D, Spain
Nano Res Appl, Volume:4
DOI: 10.21767/2471-9838-C1-009