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Pain Management 2018
Internal Medicine 2018
International Journal of Anesthesiology & Pain Medicine
ISSN: 2471-982X
Page 87
March 26-28, 2018
Vienna, Austria
JOINT EVENT
7
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Internal Medicine and Patient Care
&
6
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Pain Management
Volume 4
I
n everyday clinical practice wounds occupy an important place.
A correct and efficient approach to wound healing is important.
Various non-healing defects may be successfully treated
using a novel technique of tissue augmentation. Successful
angiogenesis forms the basis of a tissue filler survival in the
wound bed and this in turn determines the outcome of the healing
process. The aim of the study was to investigate the potential of
soft tissue fillers for wound healing of tissue defects using an
experimental in vitro model of soft tissue filler. The invasion of
endothelial cells (HUVEC) into soft tissue fillers from agarose and
collagen was studied. Standard and lowmolecular weight heparin
(concentrations of 50 IU/ml, 300 IU/ml and 2500 IU/ml) were used
as chemotactic agents. The observation intervals were 6, 12, 24,
36, 48, 60 and 72 hours, when the experiment was completed. The
observed parameters included the orientation, shape and position
of cells, the extent and distance of invasion into soft tissue filler.
It was found that agarose and collagen soft tissue fillers are
useful for further invasion studies. These observations confirm
the potential for tissue augmentation with soft tissue fillers and
may be transposed to the clinical trials with the aim of soft tissue
defects reconstruction.
tvelnar@hotmail.comSoft tissue fillers in wound healing: in vitro experiments
Tomaz Velnar
1,3
and
Lidija Gradisnik
1, 2
1
Alma Mater Europaea University Maribor, Slovenija
2
Maribor Institute for Biomedical Sciences, Slovenia
3
University Medical Centre Ljubljana, Slovenia
Int J Anesth Pain Med 2018, Volume 4
DOI: 10.21767/2471-982X-C1-003