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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 54
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
L
iver cirrhosis is a chronic disease which normal liver tissue is
replaced by fibrosis and scar leading to liver malfunction. The
purpose of this study was to induce cirrhosis in rat as an animal
model and treat cirrhosisbyMesenchymal stemcellsderived from
adipose tissue. 45 adult rats were used in this study. Group 1(30
rats) were treated by themixture of CCL4 and olive oil for 16weeks
till cirrhosis signs appeared. Group 2 (15 rats) were not treated.
Following the confirmation of cirrhosis, under anaesthesia, the
linea alba was incised, the Stem cells were injected into the Portal
vein. 5 weeks later, the rats were euthanized. Samples of liver
tissue were collected for histopathological investigation. They
were stained by H&E and Masson trichrome and studied by light
microscope. Grossly the Cirrhosis liver appeared, Nodular, Pale
and yellowish, with adhesion. Microscopic signs were: Diffused
fibrosis, fatty changes, diffused necrosis, heterogeneous hepatic
parenchyma. The clinical results of treated rats included:
Increased movements, appetite, improved behaviour and
decreased abdomen size. The histopathologic results of liver
cirrhotic rats treated by stem cells indicated: although different
stages of liver fibrosis was observed, however the structural
parenchymal lesions were not found and this indicates that liver
cells were renewing and regenerating and forming new colonies.
In conclusion Liver cirrhosis was induced by IP injection of CCL4.
The stem cells were developed from adipose tissue and cirrhotic
livers were regenerated by injection of stem cells derived from
adipose tissue in the portal vein.
Biography
Dr. Dehghani is a professor of Veterinary Surgery, Dr. Haghani is a graduate
student at the department of Surgery, Dr. Namazi is an assistant professor
of Pathology, school of Veterinary Medicine, Shiraz University. And Dr. Gha-
deri is a professor at the Cancer research centre of the Shiraz University of
Medical Science, Shiraz, Iran. His research interest includes Stem Cell and
Tissue regeneration.
sdehghan04@yahoo.comInduction of liver cirrhosis and treatment of cirrhotic liver
by mesenchymal stem cells derived from adipose tissue in rat
model
Dehghani SN
1
, Haghani I
1
, Namazi, F
2
, Ghaderi A
3
Department of Surgery (1) and pathology (2),
School of Veterinary Medicine, Shiraz University, and (3)
Cancer research centre, Shiraz University, Iran.
Dehghani SN, J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-005