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Journal of Transmitted Diseases and Immunity
ISSN: 2573-0320
Page 53
Volume 4
May 10-11, 2018
Frankfurt, Germany
Immunology Research 2018
Tissue Science 2018
JOINT EVENT
2 2
n d
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology and
Evolution of Infectious Diseases
&
1 2
t h
E d i t i o n o f I n t e r n a t i o n a l C o n f e r e n c e o n
Tissue Engineering and
Regenerative Medicine
T
he thyroid gland plays a crucial role during embryonic
development and organogenesis. It further controls the
metabolism of various adult organs. Mechanisms that drive
thyroid morphogenesis have not been fully elucidated. The inter-
follicular extra cellular matrix supports the interaction of the
functional units, the thyroid follicles, where thyroid hormone (T3 &
T4) biosynthesis takes place. The development of 3D structures
in vivo
requires cell-cell interactions and crosstalk. Thus, we
are aiming an imitation of cellular crosstalk in order to acquire
functional organoids by culturing primary human thyrocytes in a
3D environment
in vitro
. Isolated human primary thyrocytes are
expanded in monolayer culture and cells are seeded in ultra-low
attachment plates to allow aggregation and cellular interaction.
Within two weeks of
in vitro
culture, primary human thyrospheres
restore their transcriptional status similar to the native thyroid.
Based on the multi-organ-chip technology, developed by tissue
GmbH, the interaction between selected organs can bemimicked.
Due to the major impact of thyroid hormone on the metabolism,
we want to investigate the interaction and influence between
thyroid and organs such as liver or cardiac tissue by emulating
the endocrine impact. Furthermore, based on our thyroid model,
possible endocrine disruptors can be identified using our
in vitro
test system.
Biography
Özlem Vural studied Biotechnology at Technical University of Berlin. After
working as a student assistant on
in vitro
chondrogenesis in the Group of
Prof. Dr. Roland Lauster, she started her PhD studies focusing on the gen-
eration of a human 3D thyroid model for substance testing. Her research
interests include “Human organ models, tissue engineering and thyroid”.
oezlem.vural@tu-berlin.deGenerating a 3D human thyroid model
in vitro
Özlem Vural
Technical University of Berlin, Germany
Özlem Vural, J Transm Dis Immun 2018, Volume 2
DOI: 10.21767/2573-0320-C2-005