immunology-2018-scientifictracks

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Immunology 2018
J u l y 0 5 - 0 7 , 2 0 1 8
V i e n n a , A u s t r i a
Page 54
Journal of Clinical Immunology and Allergy
ISSN 2471-304X
1 5 t h I n t e r n a t i o n a l C o n f e r e n c e o n
Immunology
The aim of this study is to investigate the isolation and characterization
of biochemical properties of water-soluble extracellular polysaccharides
(WSP) isolated from a novel bacterial strain Rhizobium massiliae CA-1 and
polyhydric alcohol (PAL) isolated from Taxus cuspidata , respectively. The
primary monosaccharide composition of the WSP and PAL was determined to
be glucose by HPAEC. Interestingly, no significant amount of any other sugars
was observed, however, glycerol and xylitol were identified as the main sugar
alcohols in PAL. We evaluated immunomodulatory effects of WSP and PAL
on RAW 264.7 macrophage activation. The results showed that the WSP and
PAL dose-dependently induced the release of the pro-inflammatory cytokines
such as TNF-α and IL-6, respectively. Furthermore, treatment of RAW 264.7
cells with PAL for 24 h remarkably increased the phosphorylation levels of
ERK, p38 and JNK in a dose-dependent manner, whereas the total protein
levels of ERK (t-ERK), p38 (t-p38) and JNK (t-JNK) remained unchanged. In
addition, WSP induced nitric oxide synthase (iNOS) expression and increased
the production of nitric oxide (NO). Intriguingly, WSP remarkably increased
the mRNA expression of Toll-like receptor-2 (TLR-2) and the phosphorylation
of MAPKs (ERK, JNK and p38) in RAW 264.7 cells. Furthermore, our results
clearly demonstrate that PAL stimulates the immune response in RAW 264.7
cells through the activation of MAPKs (ERK, p38 and JNK) signaling pathway.
To the best of our knowledge, this is the first study to demonstrate the primary
structure and immune-stimulating activities of PAL from the fruit ofT.cuspidata .
In addition, WSP activates macrophages to secrete pro-inflammatory cytokines
and induces iNOS expression via the activation of the TLR-2/MAPKs signaling
pathways. Conclusively, we suggest that WSP of R. massiliae CA-1 and PAL of
T. cuspidata can be a new immunomodulatory biopolymers enhancing the early
innate immunity. Further studies of other potent biopolymers such as chitosan
and beta-glucan are under-going.
Biography
Jae Kweon Park has completed his PhD from Shimane
University in 1998, Japan, Postdoctoral and Research
Associate studies from The Johns Hopkins University, McGill
University and University of Rochester (1998~2007). He is
currently working as Professor of the Gachon Univeristy from
2011, Korea. He has published more than 50 papers in reputed
journals and has been serving as an Editorial Board Member of
the Journal of Chitin and Chitosan, Korea.
Immunostimulating activity of bio-polymers isolated from
Taxus cuspidata and a bacterial strain Rhizobium massiliae
Jae Kweon Park, You Jin Hwang and Dae Young Kim
Gachon University, Republic of Korea
Jae Kweon Park et al., Insights Allergy Asthma Bronchitis 2018, Volume: 4
DOI: 10.21767/2471-304X-C1-002