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E u r o p e a n C o n g r e s s o n
Vaccines & Vaccination
and Gynecologic Oncology
Vaccines & Vaccination and Gynecologic Oncology 2018
O c t o b e r 2 6 - 2 7 , 2 0 1 8
B u d a p e s t , H u n g a r y
Critical Care Obstetrics and Gynecology
ISSN: 2471-9803
H
uman Papillomaviruses
(HPV) are small virus non-enveloped double-stranded circular DNA. They infect epithelial cells. Many
studies have indicated that having multiple sexual partners may lead to higher HPV transmission. Thus female sex workers
(FSWs) may be at greater risk of infection. In female sex workers (FSWs), the risk of HPV infection and cervical cancer is especial-
ly high. The aim of this work is to determine the prevalence and the genotypes of HPV that circulate in female sex workers popula-
tions in Cote d’Ivoire. From Dec’ 2015 to May’ 2016, cervical samples from 350 female sex workers were tested for some HR-HPV.
HPV DNA was amplified using PGMY09/11 primers which generated 450 base pairs at the L1 region. The samples harboring HPV
DNA were genotyped using the multiplex PCR with HPV 16, 18, 31, 33, 35, 45 and 51 primers. The mean age of this population was
32.5 years. On 350 female sex workers, HPV DNA was obtained in 51.5% of the population. A total of 168 (94.38%) specimens
harboring HPV DNA were genotypes using multiplex PCR versus 5.61%, which were not genotyped using HPV 16,18, 31, 33, 35,
45 and 51 by multiplex PCR. These 168 strains permit to identify 204 strains of HPV on whom 88.69% with single infection while
11.30% with a multiple infection. Among multiple infections, 36.84% had respectively double and triple HPV infection and 26.31 %
had four HPV infections. HPV genotypes prevalence was HPV16 (22.47%), HPV18 (26.97%), HPV35 (11.23%), HPV31 and HPV33
(7.86%) respectively and HPV45 (7.30%). Any case of HPV genotype 51 was founded. The prevalence of HPV infection in female
sex workers is high. The most genotypes which circulate in female sex workers are type 16 and type 18.
ablocito@gmail.comHuman papillomavirus (HPV) infections among
female sex workers in Cote d’ivoire
Ouattara Abdoulaye
1
, Yeo Alain
2
, blavo-kouame E B
2
, Koffi
Tchibeh Franck
1
, Saraka Nguessan Daniel
2
, Marc Steben
3
,
Faye-Kette Hortense
2
and Dosso Mireille
2
1
Felix Houphouet Boigny University of Abidjan-Cote d’Ivoire
2
Pasteur Institute of Cote d’Ivoire
3
ITSS Unity National Institute of Public Health Quebec Canada
Crit Care Obst & Gyne 2018, Volume: 4
DOI:10.21767/2471-9803-C1-003