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Volume 3, Issue 4
J Clin Epigenet
ISSN: 2472-1158
Epigenetics 2017
November 06-08, 2017
EPIGENETICS & CHROMATIN
November 06-08, 2017 | Frankfurt, Germany
2
nd
International Congress on
J Clin Epigenet 2017, 3:4
DOI: 10.21767/2472-1158-C1-003
Profiling epigenomic landscapes and gene regulatory networks
Johanna K Samuelsson
Active Motif, USA
C
hromatin immunoprecipitation followed by next generation sequencing (ChIP-Seq) and other genome wide technologies
have been integral in advancing our understanding of how epigenetic phenomena are regulated and how they affect
gene expression. However, as we ask more complex questions the limitations of traditional genome-scale approaches have
motivated researchers to develop new and improved methodologies for the characterization of the epigenomic landscape.
This presentation will cover some of the progress that we and others have made in advancing the traditional ChIP-based
assays and will include a new ChIP-Seq spike-in method for improved normalization and quantitation, a unique engineered
DNA-binding molecule-mediated ChIP technology using the CRISPR system for dissecting the chromatin structure of
your genomic regions of interest, and the development of a novel transposase based ChIP assay in which each antibody is
conjugated to a barcoded transposome enabling the investigation of multiple targets within the same sample. These advanced
technologies can be applied in new fields and diverse models to gain a deeper understanding of the complex regulatory
mechanisms governing our genomes.