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Insights in Enzyme Research
ISSN: 2573-4466
E u r o S c i C o n C o n g r e s s o n
Enzymology and
Molecular Biology
A u g u s t 1 3 - 1 4 , 2 0 1 8
P a r i s , F r a n c e
Enzymology 2018
Background:
Th17 plays an important role in promoting the inflammatory response in the body, while Treg cells have the function
of inhibiting excessive inflammation. Therefore, Th17/Treg ratio plays an important part in regulating the inflammatory response.
Th17 and Treg are all differentiated from the naive CD4
+
T cells, which is mainly in the spleen. In the case of stroke, when infected,
the Th17/Treg ratio increases, but the body's immunity is reduced. Studies have shown that there is a specific expression of
microRNA in the subsets of T cells. We speculate those microRNAs that are specifically expressed in Th17 and Treg in maintaining
their polarity and characteristic cell function.
Objective:
We aimed to study the expression of specific expression of microRNA in Th17 and Treg in the case of post stroke
pulmonary infection. The effects of these microRNA on the differentiation, function and plasticity of Th17 and Treg were also
studied.
Methods:
The right MCO rats were injected
Pseudomonas aeruginosa
bacterial suspension into the trachea after 24h (the
experimental group) and in the control group, the right external carotid artery was separated and ligated, but the thrombus line
was not inserted. The bacterial suspension was injected into the trachea 24h after operation also. After 24h, the two groups of
animals were sacrificed and the spleen was taken out. The naive CD4
+
T cell were isolated by flow cytometry. The expression
level of this characteristic microRNA was detected. In
in vitro
experiments, the inhibitors and activators of differential expression
of microRNA in Th17 and Treg were applied to Th17 and Treg, respectively. Then, firstly, we use antigen to stimulate the two
subsets and measured the cell function by detecting their specific cytokine. Secondly, Treg was cultured under Th17 polarization
condition, Th17 was cultured under Treg polarization condition and the proportion of two cells and their specific cytokine were
detected by 3 days after.
Results:
Some characteristic microRNA in the two subsets expressed differently (miR-519-3P, miR-559, miR-129-3p, miR-542-
3p and miR-548). These microRNA affected the level of differentiation (miR-519-3P, miR-559 and miR-129-3p), cell function and
plasticity of Th17 (miR-519-3P) and Treg (miR-129-3p, miR-542-3p and miR-548), respectively.
Conclusion:
In the presence of stroke, the difference in expression of some microRNA can change differently, function and
plasticity of Th17 and Treg and then influence the immune function when there is pulmonary infection.
gztneurology@163.comRoles of microRNAs in Th17 and Treg in post
stroke pulmonary infection
Zhengtian Gu and Fuling Yan
Zhongda Hospital, Southeast University, Nanjing, China
Insights Enzyme Res 2018, Volume 2
DOI: 10.21767/2573-4466-C1-003