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Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
August 27-28, 2018
Zurich, Switzerland
Chemistry Education 2018
Page 14
8th Edition of International Conference on
Chemistry Education
and Research
Amyloid beta (Aβ) aggregation is generally associated with
Alzheimer’s onset. We have demonstrated that incubation
of dopaminergic SH-SY5Y cells with an Aβ peptide fragment
(an 11-mer composed of residues 25–35; Aβ (25–35)) results
in elevated intracellular nitrosative stress and induces chemical
mutation of protein disulfide isomerase (PDI), an endoplasmic
reticulum-resident oxidoreductase chaperone. Furthermore,
Aβ (25–35) provokes aggregation of both the minor and major
biomarkers of Parkinson’s disease, namely, synphilin-1 and
α-synuclein, respectively. Importantly, fluorescence studies
demonstrate that Aβ (25–35) triggers colocalization of these
Parkinsonian biomarkers to form Lewy-body-like aggregates, a
key and irreversible milestone in the neurometabolic cascade
leading to Parkinson’s disease. In addition, fluorescence assays
also reveal direct, aggregation-seeding interactions between Aβ
(25–35), PDI and α-synuclein, suggesting neuronal pathogenesis
occurs via prion-type cross-transfectivity. These data indicate
that the introduction of an Alzheimer’s-associated biomarker in
dopaminergic cells is proliferative, with the percolative effect
exercisedviadual, independent, Parkinson-pathogenicpathways,
one stress-derived and the other prion-like. The results define a
novel molecular roadmap for Parkinsonian transfectivity via an
Alzheimeric burden and reveal the involvement of PDI in amyloid
beta induced Parkinson’s. We have also explored the ability of
phytochemicals to intercept Aβ-driven Parkinson’s pathogenesis
via multiple mechanisms. Results from these studies will be
discussed.
Biography
Mahesh Narayan has completed his PhD in Biophysics at The Ohio State
University and pursued Postdoctoral studies at Cornell University. Currently,
he is a Professor of Chemistry at The UTEP and serves on the Editorial
Board ofPLOS One and Cell Biochemistry and Biophysics. He has published
extensively in the areas of protein folding, Parkinson’s disease and peda-
gogical inroads in chemistry.
Aβ, a risk factor for Parkinson’s
pathogenesis: Mechanisms and prevention
Mahesh Narayan
The University of Texas at El Paso, USA
Mahesh Narayan, J Org Inorg Chem 2018, Volume 4
DOI: 10.21767/2472-1123-C5-013