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A p r i l 1 8 - 1 9 , 2 0 1 9
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Page 28 Nano Research & Applications
ISSN: 2471-9838 Advanced Nanotechnology 2019
EuroSciCon Conference on
Advanced Nanotechnology
Bacille Calmette-Guerin (BCG) is still the only authenticated vaccine against
tuberculosis. But due to its drawbacks, a need to a new formula has
emerged. Implication of "Nanovaccinology" is one of the possible alternatives.
The non-viral vectors have low transfection ability. In the context, this work
aims to add two adjuvants to calcium phosphate nanoparticles (CPNPs)
functionalized with ESAT-6 cloned pc DNA3.1 (+) plasmid. ESAT-6 gene is
specific to Mycobacterium tuberculosis complex (MTC) and encodes a T-cell
antigen. The adjuvants in practice are Herring protamine and CpG-ODN 7909.
Each has different strategies in enhancing the immune response, protamine
is particulate adjuvant while CpG is an immunopotentiator substance.
Nanocomplex was transfected in to after THP-1 monocytic cell line its
activation to macrophage via 100ng/ml PMA. Cellular immune response, IL-12
and TNF-ɑ also ESAT-6 protein production were assayed via Sandwich ELISA
technique. Results revealed that, CPNPs offer only a partial protection to the
adsorbed plasmid against enzymatic degradation. Nanocomplex formula
with two adjuvants resulted in significantly higher cellular immune response
comparing to formula carrying one adjuvant. In conclusion, implication of
CPNPs in gene delivery accompanied with two adjuvants each possess
different strategy will result in partial protection to the delivered gene with
upsurge cellular immune response.
Biography
Dr. Ghassaq AlUbaidi, has completed her PhD in Medical
Microbiology at age of 42, from Dep. of Microbiology/ College
of Medicine/ AL-Nahrain University. The dissertation involved
the application of nanomaterials in pathogens diagnosis and
gene delivery.She has been working a biological researcher
since 2003. Now, she is working as a researcher in the Medical
research unit / College of Medicine/ AL-Nahrain University. She
is a member in the British Society for Immunology (BSI) since
2017. She has published 7 articles in fields of Virology and
nanomaterials. Now, she is actively involved in searching for a
DNA vaccine againstM. tuberculosis, which represent a serious
health problem in Middle East and African countries.
Evaluation of synergistic effect of CpG-ODN-7909 and protamine
on transfection process mediated by calcium phosphate
nanoparticles
Ghassaq T Al Ubaidi1, Ahmed A Abbas1, Ali A Taha2, Qasim S
Sharhan1 , Israa W Ahmed1 and Ilham A Jasim1
1College of Medicine, Al Nahrain University, Iraq
2University of Technology, Iraq
Ghassaq T Al Ubaidi et al., Nano Res Appl 2019, Volume:5
DOI: 10.21767/2471-9838-C2-033