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E u r o p e a n C o n g r e s s o n
Advanced Chemistry
Advanced Chemistry 2018
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
Page 34
Journal of Organic & Inorganic Chemistry
ISSN: 2472-1123
C
ellular glycans in the form of glycoconjugates are generated
by action of glycosyltransferases and glycosidases. Owing to
biological and pathological significance of glycosidases, it is highly
demanded to develop simple and sensitive methods to profile their
catalytic activities. For this purpose, carbohydrate microarrays
containing fluorescent probe-conjugated glycans were prepared by
immobilizing glycosylated near-infrared (NIR) fluorescent probes
containing hydrazide appendages on epoxide-modified glass slides
using an automatic pin-type microarrayer. Several glycosidases were
applied to the glycan microarrays and the fluorescence intensities
arising fromcleavage of a sugar moiety fromglycosylated near-infrared
(NIR) fluorescent probes weremeasured by using amicroarray scanner.
The results of microarray experiments revealed that carbohydrate
microarrays immobilized by glycosylated NIR probes are highly useful
for profiling glycosidase activities and determination of IC50 values of
glycosidase inhibitors.
Glycosylated fluorescent probe immobilized microarrays for
profiling glycosidase activities
Ji Young Hyun and Injae Shin
Yonsei University, Korea
Biography
Ji Young Hyun has received her BS degree in Chemistry in 2013 from
Yonsei University. She began her PhD with Professor Injae Shin of Yonsei
University in 2013. Her current research interests include Functional
Studies of Glycans using Synthetic Carbohydrates andGlycanMicroarrays.
hyunjy@yonsei.ac.krJi Young Hyun et al., J Org Inorg Chem 2018, Volume: 4
DOI: 10.21767/2472-1123-C2-006