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E u r o S c i C o n C o n f e r e n c e o n
PEDIATRICS
2017
Pediatrics 2017
Volume:3 Issue:4(Suppl)
Journal of Pediatric Care
ISSN 2471-805X
N o v e m b e r 1 3 - 1 4 , 2 0 1 7
L o n d o n , U K
Page 33
J Pediatr Care 2017, 3:4(Suppl)
DOI: 10.21767/2471-805X-C1-003
B
acterial meningitis continues to be an important cause
of mortality and morbidity in infants and children through
the world, and a major contributing factor is our incomplete
understanding of the pathogenesis of this disease. A high-
degree of bacteraemia and bacterial invasion of the blood-
brain barrier represent the key steps in the development of
bacterial meningitis, but the underlying mechanisms remain
incompletely understood. Using trancriptome analysis of
bacteria derived from a high-degree of bacteraemia and
meningitis, we showed that a high-degree of bacteraemia
exploits oxidative stress within bacterial cell and reducing
oxidative stress inhibits the development of bacterial
meningitis. Using proteomic arrays, RNA-Seq analysis and
CRISPR/Cas9 approaches, we showed that bacterial invasion
of the blood-brain barrier exploits specific host factors and
counteracting such exploited host factors inhibits bacterial
penetration of the blood-brain barrier as well as reduces the
Novel developments
and methodologies
for preserving child
wellbeing from
serious infection
Kwang Sik Kim
Johns Hopkins University School of Medicine,
USA
mortality and morbidity associated with bacterial meningitis.
These findings demonstrate that elucidation of the microbial
and host factors contributing to bacterial penetration of
the blood-brain barrier provides an innovative approach
for the development of prevention and therapy of bacterial
meningitis. This is the first demonstration of utilizing state
of the art methodolgies for discovery of targets for bacterial
meningitis for protecting infants and children from serious
infections
kwangkim@jhmi.edu