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Volume 3, Issue 3 (Suppl)
J Obes Eat Disord
ISSN: 2471-8203
Obesity Medicine 2017
October 30-31, 2017
October 30-31, 2017 Bangkok, Thailand
15
th
International Conference on
Obesity Medicine
Diospyros kaki
fruit and
Citrus unshiu
peel mixture (PCM) enhances fecal lipid excretion through the
inhibition of fat absorption
Seong-Soo Roh
1
and Hae Jin Park
2
1
Onbiotec Inc., South Korea
2
Daegu Haany University, South Korea
T
his study aimed to investigate the action of
Diospyros kaki
fruit (young persimmon) and
Citrus unshiu
peel mixture extract
(PCM) on the excretion of dietary triglyceride in high fat diet induced obese mice. The inhibitory activity on pancreatic
lipase of PCM was evaluated
in vitro
and its anti-obesity effects were studied based on the serum, liver and fecal triglyceride
level analysis from high-fat diet (HFD)-fed mice
in vivo
. Moreover, we measured the protein expressions of AMP-activated
protein kinase alpha (AMPKa), the fatty acid oxidation and thermogenesis-related genes (e.g.,
PPARα
,
CPT-1
and
UCP-2
)
and lipogenesis-related genes (e.g.,
SREBP-1, SREBP-2, ACC, SCD-1
and
HMGCR
) with western blot in the liver. Hepatic
functional parameters were analyzed in the serum. PCM exhibited the inhibitory effect on lipase activity with an IC50 value
of 428.9 μg/ml. Moreover, the dietary TG by the PCM200 treatment was significantly excreted through the feces. Furthermore,
PCM200 treated mice increased significantly the level of phosphorylated AMPKα in obese mice. Lipogenesis-related protein
expressions in PCM200 were marked reduced compared to these of HFD control mice, whereas the PCM administration did
not show any effect on fatty acid oxidation and thermogenesis related protein expressions. Thus, the PCM treatment produced
no obvious adverse effects on the liver function. Taken together, PCM promoted fecal lipid excretion by inhibiting intestinal
absorption of dietary fat and improving lipid metabolism
via
activation of AMPK. Accordingly, these data clearly show that
PCM possess an anti-obesity function in mice fed with HFD. Accordingly, PCM may be a promising herbal formula in the
prevention or treatment of obesity.
Biography
Seong-Soo Roh has lectured and studied Pharmacology and Toxicity in Korean Medicine at Daegu Haany University, South Korea. In addition to natural drug
research, he also has focus on research of functional food ingredients and natural cosmetic materials. Presently he is the Editor-In-Chief of The Korea Association
of Herbology and serves as an Academic Member and Editor of internationally renowned journals.
ddede@dhu.ac.krSeong-Soo Roh et al., J Obes Eat Disord 2017, 3:3
DOI: 10.21767/2471-8203-C1-005