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A n n u a l C o n g r e s s o n
Nursing & Healthcare
December 03-04 , 2018
Amsterdam, Nether l ands
Journal of Nursing and Health Studies
ISSN: 2574-2825
Nursing & Healthcare 2018
Main Objective: To evaluate the performance of GeneXpert MTB/RIF in detection of pulmonary TB and drug resistant testing.
Specific Objectives:
To determine the relationship between TB prevalence and the risk factors of gender and age; determine the
performance of GeneXpert MTB/RIF; evaluate the effectiveness of the assay in smear positive and smear negative pulmonary
specimens and determine the operational feasibility and cost effectiveness of GeneXpert MTB/RIF in routine use.
Design:
Descriptive cross-sectional study.
Setting:
Kitui County Hospital.
Study subjects:
Adult patients of 18 years and above presenting to Kitui County hospital with symptoms suggestive of pulmonary
tuberculosis or multi-drug resistant tuberculosis.
Main outcome measures:
Smear microscopy test method had the highest number of false positives (28%) and false negatives
(9.6%).
Results:
400 samples were analyzed, 37.5% were smear positive of which 60% (p<0.05) were male. For culture and GeneXpert,
the positive samples were 33% and 32.25% respectively. Smear microscopy had the highest number of false positives (28%)
and false negatives (9.6%). For bacilli identification, the sensitivity, specificity, positive predictive value and negative predictive
values for smear microscopy were 81.8%, 84.3%, 72% and 90.4%; GeneXpert were 97.7%, 100%, 100% and 98.9% respectively.
Drug susceptibility testing using culture method showed 23 isolates were rifampicin resistant with GeneXpert, they were 26,
implying 3 false positives. The sensitivity, specificity, positive predictive value and negative predictive value for GeneXpert in drug
susceptibility testing was 100%, 97%, 89% and 100%. Cost of testing samples with GeneXpert assay was high, but offers rapid
detection.
Conclusion:
GeneXpert MTB/RIF offers high potential for rapid diagnosis of TB and drug susceptibility testing.
Recommendation:
GeneXpert be considered for routine drug susceptibility testing of TB samples.
paulofs@unb.brPerformance of GeneXpert test in detecting PTB
and rifampicin resistance in patients attending
Kitui County Hospital
Prominah Kavinya Muia
Kenya Medical Training College, Kenya
J Nurs Health Stud 2018, Volume: 3
DOI: 10.21767/2574-2825-C5-015