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Volume 3, Issue 4 (Suppl)
Nano Res Appl
ISSN: 2471-9838
November 07-08, 2017 Singapore
International Meeting on
Advanced Nanomaterials and Nanotechnology
Nanomaterials Meetings 2017
November 07-08, 2017
Page 47
Nano Res Appl 2017, 3:4
DOI: 10.21767/2471-9838-C1-006
Phospholipid stabilized gold nanorods: Towards improved colloidal stability and biocompatibility
Poornima Budime Santhosh
a
, Neethu Thomas
a
, Swathi Sudhakar
a
, Anju Chadha
b,c*
, Ethayaraja Mani
a*
PolyIndian Institute of Technology Madras, India
B
iocompatible and colloidally stable gold nanorods (GNRs) of well-defined plasmonic properties are essential for biomedical and
ther-anostic applications. The as-synthesized GNRs in the seed-mediated method are stabilized by cetyltrimethylammonium
bromide (CTAB) surfactant, which is known for its cytotoxicity in many cell lines. Bio-compatible GNRs synthesized from known
protocols exhibit some extent of cytotoxicity and colloidal instability because of incomplete removal of CTAB. We report a facile
method for the efficient re-moval of CTAB molecules with 1,2-dimyristoyl-sn-glycero-3-phos-phocholine (DMPC) phospholipids,
which are naturally present in cell membranes. The ligand exchange kinetics is studied using surface-enhanced Raman scattering
(SERS) and corroborated with matrix assisted laser desorption/ionization (MALDI) mass spectrometry. From colloidal stability
studies using dynamic light scattering (DLS) and UV-Vis spectroscopy, the optimal lipid concentration and dura-tion required
for successful ligand exchange of CTAB by DMPC are reported. Using thermogravimetric analysis, the surface concentra-tion of
DMPC on colloidally stable GNRs is found to be approxi-mately 9 molecules per nm2. The 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl
tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays show that the surface modified DMPC-GNRs have significantly
better biocompatibility compared to CTAB-GNRs. Stud-ies on ligand exchange, colloidal stability and biocompatibility of DMPC-
GNRs of aspect ratios in the range of 2.2 - 4.2 demonstrate the robustness of the proposed method. The results provide insight on the
important factors to be considered to design biocompatible GNRs suitable for applications in nanomedicine.
poorni_balaji@yahoo.com