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Nanobiotechnology 2018
Page 42
Nano Research & Applications
ISSN: 2471-9838
E u r o S c i C o n C o n f e r e n c e o n
Nanotech & Nanobiotechnology
J u l y 1 2 - 1 3 , 2 0 1 8
P a r i s , F r a n c e
T
his study aimed to investigate howmesoporous silica nanoparticles
(MSNs), especially focusing on their surface functional groups,
interacted with zebrafish embryos and performed the penetration
into blood-brain barrier. Surface properties, such as size, charge and
surface chemistry, are a particularly important determinant influencing
the biological fate and actions between the nanoparticles and cells.
Eight kinds MSNs were synthesized with the uniform and mesoporous
structure in ranged from+35.7 to 46.8mV of zeta potential and in size of
50nm or 200nm. By pericardial injection into 72 hpf zebrafsh embryos,
results observed were plenty of N4-MSN@PEG/THPMP
50
entering into
larval brain; N1-MSN@PEG
50
had some; however, particles in positively
charged were hardly found in the brain. It indicated that particles in
negatively charged can penetrate blood-brain barrier into larval brain
area. The confocal image was also confrmed by the two-proton image.
The confocal image of all three N2, N3 and N5-MSN@PEG/THPMP
50
particles clearly presented in the larval brain area in similar pattern as
N4-MSN@PEG/THPMP
50
. However, the N4-MSN@PEG/THPMP
200
had
not shown the penetration effect in the brain. The results illustrated
that the brain penetration effect is may due to a negatively charged
dependent and size-dependent manner.
Negatively charged mesoporous silica nanoparticles
penetrate through the Zebrafish larval blood-brain barrier
Chien-Tsu Chen
1
, Yi-Ping Chen
2
, Si-Han Wu
2
, Tsu-Yuan Chang
1
and Chih-Ming Chou
1
Taipei Medical University, Taiwan
Biography
Chien-Tsu Chen is a Professor of Department of Biochemistry and Cell
Biology at School of Medicine, Taipei Medical University. His academic
and research expertise include Nanotechnology, genetic engineering,
nanomedicine, protein therapeutics, health promotion, antibody therapy
Nanotechnology, Genetic engineering, Nanomedicine, Protein therapy,
Health promotion, Antibody therapy. He completed his PhD in 1993 from
Brandeis University, WalthamMA and Visiting Scholar in 2006 at University
of Washington, Seattle WA. He was the President of St. Mary Medicine,
Nursing and Management College in 2007.
chenctsu@tmu.edu.twChien-Tsu Chen et al., Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C2-012