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Dentistry and Craniofacial Research
ISSN: 2576-392X
3
rd
Euroscicon Conference on
M a r c h 2 5 - 2 6 , 2 0 1 9
B u d a p e s t , H u n g a r y
Dental Hygiene 2019
Dental & Dental Hygiene
Aims:
To investigate changes in calcitonin gene-related peptide (CGRP)-like immunoreactivity (CGRP-LI) in the rat masseter
muscle and brain after the unilateral experimental induction of masseter myositis.
Methods:
Ipsilateral and contralateral changes of the CGRP were examined in rat masseter muscle after the induction of unilateral
myositis on the right side with an intramuscular injection of 0.01 mL Freund's adjuvant. The left masseter, and left and right
masseters of control rats, were injected with 0.01 mL saline (0.9%). After 21 days, tissue samples from the masseter muscles
and the hypothalamic-pituitary-adrenal (HPA) axis were analysed for the presence of CGRP by immunohistochemistry, radioactive
immunoassay, and high-performance liquid chromatography. Haematoxylin-eosin staining was used to confirm inflammation in
the masseter muscles.
Results:
ElevatedCGRP-LI was detected bilaterally in themassetermuscles (P < .001) in themyositis group. CGRP-immunoreactive
nerve fibres were mainly detected in close proximity to muscle cells and in the walls of the blood vessels. Compared to the control
rats, a significant difference in scratching behaviour was seen in the myositis group from day 9 until day 21. In the myositis
group, CGRP-LI was increased in the pituitary gland concomitant with the increase in CGRP-LI in the masseter muscles but was
decreased in the hypothalamus. A possible explanation for these changes could be that rats with chronic myositis develop an
abnormal function of the HPA axis triggered by masseter muscle inflammation.
Conclusion:
The results of this study demonstrate that CGRP may play an important role both peripherally and centrally in
masseter muscle myositis in association with presumed nociceptive behaviour.
Joakimwaldemarson@gmail.comCALCITONIN GENE-RELATED PEPTIDE CHANGES IN
MUSCLE AND BRAIN WITH EXPERIMENTALLY INDUCED
UNILATERAL RAT MASSETER MYOSITIS
Joakim A Waldemarson
Karolinska Institute, Sweden
Dent Craniofac Res 2019, Volume:4
DOI: 10.21767/2576-392X-C2-018