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Volume 2, Issue 2 (Suppl)
Chronic Obstructive Pulmonary Diseases
ISSN: 2572-5548
Page 53
conferenceseries
.com
CO-ORGANIZED EVENT
August 31-September 01, 2017 Brussels, Belgium
&
International Conference on
Chronic Diseases
6
th
International Conference on
Microbial Physiology and Genomics
Detection of JC Polyomavirus Tumor-Antigen In Gastric Carcinoma, a report from IRAN
Farnaz Zahedi Avval
1,2
, Samira Izi
1
, Farzad Rahmani
1
, Nema Mohammadian Roshan
3
, Atefeh Yari
4
, Masoud Youssefi
4,5
1
Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
2
Metabolic Syndrome Research Center, Nutrition and Biochemistry division, Mashhad University of Medical Sciences, Mashhad, IRAN
3
Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
4
Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
5
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IRAN
Background:
Although the global incidence of gastric cancer has been declined dramatically over the recent few decades, it
is still a common cancer in different parts of the world. It has been scientifically suggested that some oncogenic viruses like
JCV might play a role in Gastric carcinogenesis. The viral transforming protein, T-antigen (T-Ag) has the ability to bind and
inactivate tumor suppressor proteins including p53 and pRb that might lead to malignant outcome, though its role might vary
in different geographic parts due to viral distribution and other habits resulting in GI cancer.
Objective:
The aim of present study was to investigate the presence of JCV T-Ag sequence and its expression in cancerous and
non-cancerous adjacent gastric tissues in Iranian patients.
Methods:
Thirty one sample pairs of formalin fixed paraffin embedded (FFPE) tissue specimens of gastric cancer and adjacent
non-cancerous tissues (ANCT) were investigated on the basis of Real-time polymerase chain reaction. Samples were subjected
for the immunohistochemistry examination using an anti-T-Ag monoclonal antibody.
Results:
Real time experience followed by sequencing revealed JCV sequences in 17 (54.84%) of gastric cancer tissues and in
10 (32.25%) of non-cancerous gastric mucosa (OR=1.7). Immunohistochemical study also showed the presence of T-Ag in
nuclear compartment.
Conclusions:
These data indicate, for the first time, presence of JC virus in gastric carcinoma samples in our socioeconomic
region. These findings provide a summative, supportive data for a possible role of JCV T-Ag in carcinogenesis of gastric
malignancy.
ZahediAF@mums.ac.irFarnaz Zahedi Avval et al., Chron Obstruct Pulmon Dis 2017, 2:2
DOI: 10.21767/2572-5548-C1-003