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Volume 4
December 10-12, 2018
Rome, Italy
Nano Research & Applications
ISSN: 2471-9838
Advanced Materials 2018
Nano Engineering 2018
JOINT EVENT
22
nd
International Conference on
Advanced Materials
and Simulation
&
22
nd
Edition of International Conference on
Nano Engineering &
Technology
I
n recent years, nanotechnology has become a key player
in multiple biomedical fields, drug delivery being one of the
domains where nanotechnological innovations are highly applied.
Nanocarrier systems proved to be very useful in improving the
physicochemical and pharmacological properties of different
compounds, thus augmenting their effectiveness as therapeutic
agents. Betulinic acid (BA) is a pentacyclic lupane-type
triterpene of natural origin that exerts a plethora of biological
effects, including antitumor, antiviral, anti-inflammatory,
immunomodulatory, anti-angiogenic, hepatoprotective, etc.
The main handicap of BA consists in very low water solubility
what limits its use
in vivo.
To adjust this flaw and to improve its
bioavailability, we prepared a nanoformulation of BA using silver
and silver PEGylated (PEG) nanoparticles and verified its cytotoxic
effects invitroagainst apanel of tumor cell lines, as: human (A375)
and murine melanoma (B164A5), lung (A549), breast (MCF-
7 and MDA-MB-231) and hepatic carcinoma (HepG2) and on a
healthy cell line – HaCaT – human immortalized keratinocytes.
The obtained nanoformulation were characterized in terms
of physicochemical properties by applying standard methods
as transmission electron microscopy (TEM) and UV-VIS, that
confirmed the development of stable solutions. The cytotoxicity
was evaluated by the means of MTT (3-(4,5-Dimethylthiazol-2-yl)-
2,5-Diphenyltetrazolium Bromide) and Alamar blue techniques,
and the impact on cell migration and proliferation was measured
using scratch assay. The nanoformulations of BA managed to
inhibit the proliferation of all tumor cells at a higher extent as
compared with the solution of BA in DMSO (dimethyl sulfoxide)
used as the standard, whereas in the case of a healthy cell line,
the toxic effect was minimum. The migration of tumor cells was
also impaired by the nanoformulations. These preliminary results
indicate that the antiproliferative effect of BA was improved.
Further studies are required to establish the mechanism of action
of this nanoformulation and to prove its effectiveness
in vivo
.
Recent Publications
1. Coricovac D, Dehelean C, Moaca EA, Pinzaru I, Bratu T,
Navolan D and Boruga O (2018) Cutaneous melanoma-a
long road from experimental models to clinical outcome:
a review. International Journal of Molecular Sciences
19(6):E1566.
2. Pinzaru I, Coricovac D, Dehelean C, Moacă EA, Mioc
M, Baderca F, Sizemore I, Brittle S, Marti D, Calina C D,
Tsatsakis A M and Şoica C (2018) Stable PEG-coated
silver nanoparticles - A comprehensive toxicological
profile. Food and Chemical Toxicology 111:546-556.
3. CoricovacDE,MoacăEA, Pinzaru I, CîtuC, SoicaC,Mihali
C V, Păcurariu C, Tutelyan V A, Tsatsakis A and Dehelean
C A (2017) Biocompatible colloidal suspensions based
on magnetic iron oxide nanoparticles: synthesis,
characterization and toxicological profile. Frontiers in
Pharmacology 8:154.
4. Gheorgheosu (Coricovac) D, Duicu O, Dehelean C, Soica
C and Muntean D (2014) Betulinic acid as a potent and
complex antitumor phytochemical: a minireview. Anti-
Cancer Agents in Medicinal Chemistry 14(7):936-45.
5. Gheorgheosu (Coricovac) D, Jung M, Ören B, Schmid T,
Dehelean C, Muntean D, Brüne B (2013) Betulinic acid
suppresses NGAL-induced epithelial-to-mesenchymal
transition in melanoma. Journal of Biological Chemistry
394(6):773-81.
Dorina E Coricovac
and
Cristina A Dehelean
Victor Babes University of Medicine and Pharmacy Timisoara, Romania
Dorina E Coricovac et al., Nano Res Appl 2018, Volume 4
DOI: 10.21767/2471-9838-C7-028
Functionalization of betulinic acid by
nanotechnology improved its
in vitro
antiproliferative activity