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Acupuncture Congress 2018 Herbal Medicine: Open Access
ISSN: 2472-0151 Page 50
August 20-21, 2018
Dublin, Ireland
9th International Conference on
Acupuncture &
Chinese Medicine
The unfavorable microenvironment of the injured spinal cord
results in the low cell survival rate and astrocytic differentiation
of the grafted neural stem cells (NSCs). Our previous study found
that electroacupuncture (EA) on the governor vessel acupoints
enhanced neurotrophin-3 (NT-3) level in the injury/graft site of
spinal cord and promoted the survival and migration of NSCs
grafted immediately. However, the transplanted NSCs appeared
low efficiency in differentiating into neurons. The NSC derived
neurons are especially needed for reconstructing circuit of the
damaged spinal cord. Therefore, in the present study, first in vitro,
we attempted to co-culture withNT-3 genemodified NSCs andTrkC
(receptor of NT-3) gene modified NSCs in a gelatin sponge scaffold
(GS) for 14 days, to establish a neural network scaffold (NN) with
synaptic transmission and then, the NN was transplanted into a
2 mm wide transection gap in the T9-10 spinal cord of adult rats,
to investigate whether EA application could promote the survival
and function of transplanted NN by enhancing the secretion of
endogenousNT-3 in the injured spinal cord. The results showed that
EA treatment can increase NT-3 levels of the injured spinal cord,
maintain the survival, differentiation and synaptic connections of
the NSC derived neurons in the grafted NN, and encourage axonal
regeneration in the injury/graft site of spinal cord, as well as
improve neural transduction of spinal cord and paralysis hindlimb
function. Our data suggest that the combinational strategy of EA
and grafted NN can partially improve the structure and function of
transected spinal cord.
Biography
Ying Ding is an Associate Professor, Master’s Supervisor in the Department
of Histology and Embryology from Zhongshan School of Medicine at Sun
Yat-sen University. She is a Member of Guangdong Provincial Anatomical
Society Council; Guangdong Provincial Rehabilitation Medicine Association
and Guangdong Provincial Experimental Medicine Professional Committee
of Chinese and Western Medicine Association. Her research mainly focuses
on the mechanism of electroacupuncture combined with transplanted stem
cell repairing spinal cord injury. So far, she has obtained some grants from
National Natural Science Foundation and the Natural Science Foundation of
Guangdong Province. She has totally published 23 SCI-E papers.
Ying Ding, Hui Jin and Yuan-Shan Zeng
Sun Yat-sen University, China
Ying Ding et al., Herb Med. 2018, Volume 4
DOI: 10.21767/2472-0151-C1-003
Electroacupuncture maintains the survival and synaptic
connection of neural stem cell-derived neurons in a grafted
neural network scaffold via enhancing neurotrophin-3
secretion, and improves neural transduction in the transected
rat spinal cord