Journal of Molecular and Cellular Biochemistry

Polymerase chain reaction (PCR) in molecular biology is used to amplify a single copy or a few copies of a DNA segment across several orders of magnitude, it can generate thousands to millions copies of a particular DNA sequence. PCR amplifies a specific region of a DNA strand called the DNA target. Most PCR methods amplify DNA fragments of between 0.1 and 10 kilo base pairs (kbp), The amount of amplified product is determined by the substrates in the reaction. Applications of polymer Chain Reaction- Selective DNA isolation, Amplification and quantification of DNA, genetic testing, tissue typing, leukemia, lymphomas, human immunodeficiency virus, tuberculosis and genetic fingerprinting.

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