Insights in Enzyme Research

The probable effect of MT1A (A>G) and MT1A (C>G) SNPS of metallothionein gene on whole blood mercury levels in Iranian populations

EuroSciCon Congress on Enzymology and Molecular Biology
August 13-14, 2018 Paris ,France

Mehdi Abdi

Valiasr Hospital, Tehran, Iran

Posters & Accepted Abstracts: Insights Enzyme Res

DOI: 10.21767/2573-4466-C1-003

Abstract

Polymorphism in metalloproteins may lead to changes in heavy metal levels in the body. The risk factors of polymorphisms in heavy metal concentrations, particularly mercury, may be due to several confounding factors including differences in ethnicity of the analyzes populations, sample size and the type of the studied environment heavy metals to which population are exposed. We study the effect of MT1A (A>G) and MT1A (C>G) polymorphisms on blood mercury level in Iranian population. 300 non exposure people to control group and 150 exposure people to case group were used. DNA extraction and PCR-RFLP (restriction fragment length polymorphism) and DNA sequencing was done and blood mercury level was measured via atomic absorption spectroscopy (AAS) technique by DMA-80. Blood mercury concentration in case group was higher than control group (p value<0.001). There was no significant differences in case and control groups to the effect of MT1A (A>G) and MT1A (C>G) polymorphism on blood mercury levels and P value were 0.69 and 0.44, 0.59 and 0.56 for case and control groups, respectively. MT1A (A>G) and MT1A (C>G) polymorphism were not associated with increased level of mercury concentration in Iranian, which needs further investigations. In conclusion, this study suggest that MT1A (A>G) and MT1A (C>G) polymorphisms are not attractive susceptibility markers for high blood mercury concentration.

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