Dentistry and Craniofacial Research

The effect of atorvastatin on glucocorticoidinduced osteoporosis on periodontal bone loss

EuroSciCon Conference on Dental & Dental Hygiene
March 26-27, 2018 Edinburgh, Scotland

Paula Goes, Luzia Heminia de Sousa, Eveline Linhares, Joana Trycia Alexandre, Mario Roberto Lisboa, Mirna Marques, Helliada Vasconcelos, Conceicao Martins and Gerly Anne de Castro Brito

Federal University of Ceara, School of Medicine, Brazil Federal University of Ceara, Sobral, School of Medicine, Brazil Federal University of Ceara, School of Medicine, Brazil

Posters & Accepted Abstracts: J Den Craniofac Res

DOI: 10.21767/2576-392X-C1-003

Abstract

Background: Atorvastatin (ATV) has shown pleiotropic effects on bone tissue, and osteoporosis can aggravate periodontitis. Thus, we assessed the effects of ATV on experimental periodontitis (EP) of rats subjected to glucocorticoid-induced osteoporosis (GIOP). Methodology: Male Wistar rats were divided into: Na�?�?�?¯ve, EP, GIOP+EP and ATV groups. GIOP+EP and ATV received 7 mg/ kg of dexamethasone intramuscularly 1x/week for 5 weeks, the others received Saline (SAL). EP, GIOP+EP and ATV were submitted to EP by ligature around 2nd upper left molars for 11 days. ATV received 27 mg/kg of ATV orally and the others SAL, 30 minutes before EP. Periodontium was analyzed by macroscopy, micro-tomography and histopathology; by immunohistochemical examination of RANKL, OPG, WNT10b, DKK-1 and �?�?�?²-catenin and by ELISA analysis of myeloperoxidase (MPO), TNF-�?�?�?±, IL-1�?�?�?², -6, -8, and -10, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Leukogram and liver and kidney enzymes and bone-specific alkaline phosphatase (BALP) serum levels were performed. Results: ATV decreased bone loss, reduced MPO, TNF-�?�?�?±, IL1�?�?�?², -6, and -8, and increased IL-10, GSH, SOD and CAT levels. ATV reduced RANKL and DKK-1, increased OPG, WNT10b and �?�?�?²-catenin expressions and BALP activity. Conclusions: ATV reduced inflammation, oxidative stress and bone loss in rats with EP and GIOP, with participation of WNT signaling pathway. paulagpinheiro@yahoo.com.br