Journal of Organic & Inorganic Chemistry

Simultaneous determination of five isosteroidal alkaloids in Siberian fritillary bulb in rat plasma and its application in pharmacokinetic study by HPLC-MS/MS

Annual Congress on Medicinal Chemistry, Pharmacology and toxicology
July 30 - 31 , 2018 Amsterdam , Netherlands

Min Liang, Jianzhong Wang, Yanping Liu and Liming Ye

West China School of Pharmacy, Sichuan University, China

Posters & Accepted Abstracts: J Org Inorg Chem

DOI: 10.21767/2472-1123-C3-009

Abstract

In this study, a sensitive high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method established for simultaneously determining five main isosteroidal alkaloids (imperialine-3-β-Dglucoside (imperialine-G), imperialine, peimine, hupehenine and yibeinoside A) in Siberian fritillary bulb, was applied to pharmacokinetic studies in rat plasma. The plasma samples pretreated using liquid-liquid extraction with ethyl acetate were quantitated by multiple reaction monitoring (MRM) via positive electrospray ionization (ESI) mode. Chromatographic separation was performed on an Intersil ODS-2 column (5 μm, 4.6 ×150 mm) with a single fifteen minutes run using gradient elution. The mobile phrase consisted of (A) 10 mM ammonium acetate (containing 0.1% of formic acid) and (B) methanol. Method validation results showed that the developed method had good accuracy and precision over the corresponding linearity range for all the analytes. Besides, benchtop, autosampler, freeze-thaw circulation and long-term storage stabilities met the acceptable limit. This study examined a specific and robust method which was successfully applied to analyze rat plasma samples for pharmacokinetic study of five isosteroidal alkalosids