American Journal of Computer Science and Information Technology

Selection of poly(lactic acid) scaffolds design for triple negative breast cancer 3d culture

Euroscicon Conference on 3D Printing and Wireless Technology
September 17-18 , 2018 Lisbon , Portugal

Emma Polonio Alcala, M Rabionet, A J Guerra, J Ciurana and T Puig

University of Girona, Spain GREP-University of Girona, Spain

ScientificTracks Abstracts: Am J Compt Sci Inform Technol

DOI: 10.21767/2349-3917-C2-005

Abstract

Cells are traditionally cultured in vitro in two dimensions (2D), but it presents some limitations, as loss of their in vivo morphology and reduction of cellcell and cell-extracellular matrix (ECM) interactions. Hence, three-dimensional (3D) cell culture models, such as scaffolds, are being developed to overcome the aforementioned restrictions and to mimic the physiological tissue environment. Cancer stem cells (CSCs) are a subpopulation of a tumor or cancer cell line, which are responsible for metastasis and tumor recurrence. They have the capacity to initiate tumor growth and maintain long term self-renewal, as well as they exhibit resistance to conventional therapies. Their study in 2D culture is limited due to the induction of differentiation during CSCs propagation. Interestingly, 3D culture systems can avoid it, besides they can produce an enrichment of CSCs population. In the present study, scaffolds were manufactured by Fused Filament Fabrication (FFF) technique with the BCN3D Sigma 3D printer. Five fabrication parameters were selected to obtain 27 scaffolds designs, which have different pore size and filament diameter. The tested parameters were layer height, infill density, infill pattern, infill direction and material flow. Poly (ε-caprolactone) (PCL) was the material used to manufacture scaffolds. PCL is commonly used for tissue engineering applications due to its good mechanical characteristics, as its low melting temperature and low biodegradability, good biocompatibility, FDA approval and low cost. MDA-MB-231 triple negative breast cancer (TNBC) cells were cultured on FFF scaffolds to analyze cell efficiency. Therefore, fabrication parameters of scaffolds with highest cell proliferation rates were chosen to accommodate 3D cancer cell culture and further analyze the CSCs enrichment. The enrichment of this malignant subpopulation would facilitate future experiments to find and develop new therapeutic strategies against CSCs.

Biography

Emma Polonio Alcalá has completed her Bachelor’s degree in Biotechnology and Master’s degree in Molecular Biology and Biomedicine, from University of Girona. Her Bachelors’ degree project was regarding A New Synthetic Inhibitor of the Fatty Acid Synthase (Fasn) with Cytotoxic Effects and her Master’s project was entitled Effects of the New Antitumoral Drug Abtl0812 in Preclinical Models of Triple Negative Breast Cancer. She has performed both the projects in New Therapeutic Targets Laboratory (Targets Lab) research group in the Department of Medical Sciences from University of Girona and scored excellent marks. Some of her results were published in a congress proceeding (Giro- Perafita et al., 2017). Nowadays her research is focused on The design and fabrication of biocompatible scaffolds for three-dimensional breast cancer cell culture, in collaboration with the Product, Process and Production Engineering Research Group from University of Girona and is also linked with Targets Lab.

E-mail: emma.polonio@udg.edu