American Journal of Ethnomedicine

Safety and toxicity evaluations of Xanthium strumarium Linn

6^th Edition of International Conference on Pharmacognosy and Medicinal Plants
April 16-17, 2018 Amsterdam, Netherlands

Bhanu P S Sagar and Srishti Singh

IEC Group of Institutions, India

ScientificTracks Abstracts: Am J Ethnomed

DOI: 10.21767/2348-9502-C1-005

Abstract

Xanthium strumarium L. is poisonous to mammals due its toxic principle which is a diterpenoid glycoside i.e., atractyloside found in the roots and seeds. It was thought worthwhile to carry out the hepatotoxic assessments and safety and toxicity evaluations of oral administration of atractyloside and methanolic extracts of X. strumarium L. in albino Wistar rats. So, the present investigation was undertaken with following objectives: to develop standardized protocols for extraction, isolation, purification, characterization and quantitative estimation of atractyloside; hepatotoxic assessments of oral administration of atractyloside in albino Wistar rats and to study the safety and toxicity evaluation of methanolic extract in albino Wistar rats. Xanthium strumarium Linn. roots and seeds were found to contain alkaloids, anthraquinones, flavonoids, atractyloside, phenolics, steroids, terpenoids, and resin etc. In the present investigation, an attempt was made to separate the atractyloside by using instant preparative thin layer chromatography (IPTLC) technique. Purified atractyloside was chemically characterized by IR, mass and NMR spectral analysis. Atractyloside concentrations were found to be 2.9 and 4.3 mg/ml in plant root and seeds respectively using HPLC techniques. During hepatotoxic assessment, atractyloside produced severe hepatotoxicity in albino Wistar rats. Observations of the sub-acute and acute toxicity studies had indicated that methanolic extract of X. strumarium had shown a narrow safety margin in animals. On the basis of sub-acute and acute toxicity evaluation studies, it was established that both atractyloside and methanolic extract of X. strumarium L. possess a narrow safety margin in rats used in in vivo experimental and pre-clinical pharmacological studies.

Biography

Bhanu P S Sagar completed his PhD from Jamia Hamdard, Postdoctorate from National Institute of Immunology .Presently, he is the Director of Pharmacy College at IEC-GI and Former Vice-Chancellor of IEC University. He has published 47 papers and presented 30 papers. He has also presented two papers in AAPS 2006, National Biotechnology Conference in Boston, USA. He is the Reviewer for various international journals and was also selected for “Marquis Who’s Who in Asia” and “Marquis Who’s Who in World”. He has received many awards in his prime areas of research include plant tissue culture, phytochemical and pharmacological investigations of natural products

E-mail: bpssagar@gmail.com