Journal of Clinical Immunology and Allergy

Regulation of chromium (III) and nickel (II) of RNA virus replication

15 ^th International Conference on Immunology
July 05- 07 , 2018 Vienna , Austria

Sylwia TerpiÃ?Â?owska and Andrzej K Siwicki

The John Paul II Catholic University of Lublin, Poland University Warmia and Mazury in Olsztyn, Poland

Posters & Accepted Abstracts: Insights Allergy Asthma Bronchitis

DOI: 10.21767/2471-304X-C1-003

Abstract

The transition elements: chromium and nickel are essential micronutrients for human, animals and plants. Micronutrients in human and animal organisms play crucial role in prevention and treatment of various diseases. They also play an important role in the optimization of physical and mental functions. Inside the cells, chromium (III) may interact with microfilaments, mitochondria, lysosomes and nucleus. Cr (III) compounds can bind directly to DNA in vitro, forming Cr-DNA adducts and DNA-DNA crosslinks. Nickel can induce genotoxic effects, such as DNA strand breaks, sister chromatin exchanges and DNAprotein crosslinks. Moreover, nickel can generate ROS production, which interacts with nucleic acids or proteins. The aim of this study was to examine the effect of treating of chromium (III) and nickel (II) and their combinations on Bovine Viral Diarrhoea Virus (BVDV) replication. The BVDV has been a good model virus for investigating HCV, which is a member of genus Hepacivirus, which belongs to the same family. The antiviral efficacies of chromium (III) and nickel (II) on BVDV were evaluated using Real Time PCR method. Moreover, the cytotoxicity of these microelements was examined using the MTT reduction assay. The IC50 (50% inhibitory concentration) for the chromium chloride was 1400 μM for BT cells. The IC50 for the nickel chloride was more than 1200 μM for BT cells. The concentration-dependent antiviral activity of chromium chloride and nickel chloride against BVDV was observed. In cultures simultaneously treated with 1) 200 μM of CrCl3 and 1000 μM of NiCl2, 2) 1000 μM of CrCl3 and 200 μM of NiCl2, a decrease in number of RNA copies was observed compared with control cells and cells incubated with chromium(III) and iron(III) used separately. The synergistic antiviral effects were observed for chromium (III) and nickel (III) against BVDV.

Biography

Professor Andrzej K. Siwicki, VMD, Ph.D., DSc is a head of Department Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury and Department of Pathology and Immunology IFI in Olsztyn, Poland. He is author of over 500 original papers in reputed journals and about 200 scientific communications (index-h 32, index of citation: 4502). His fields of interest are modulation of defence mechanisms and protection against diseases by natural and synthetic products in animals, influence of pollutants on the cell-mediated immunity and restoration of immunity after suppression induced by xenobiotics. Developed a new possibility in the comparative immunotoxicology for control of effect of xenobiotics and pharmaceutical products on the defence mechanisms and protection against diseases. He attended scientific missions in USA, Japan, France and Israel, was a co-ordinator of USDA and FAO projects and he is professor conferring of 14 PhDs and 4 DSc.v.

E-mail: siwicki@uwm.edu.pl