Journal of Clinical Immunology and Allergy

Molecular identification of ESBL-producing gene blaTEM in MRSA and meats isolated bacteria

15 ^th International Conference on Immunology
July 05- 07 , 2018 Vienna , Austria

Yuan Li, Yong Hyun Lee, Seok Chan Hong, Dae Young Kim, Jae Kweon Park and You Jin Hwang

Gachon University, South Korea

Posters & Accepted Abstracts: Insights Allergy Asthma Bronchitis

DOI: 10.21767/2471-304X-C1-003

Abstract

Staphylococcus aureus is one of the most pathogenic organisms which cause infections in both healthcare and community settings. The proportion of methicillin-resistant S. aureus (MRSA) in healthcareassociated (HA) isolates was very high in South Korea. In this study, antimicrobial resistance profiles of S. aureus and meats isolated bacteria were determined by disc diffusion method. PCR was applied for detecting the presence of antibiotic resistance genes and blaTEM types. In total of 18 S. aureus and 36 meats isolated bacterias. All strains carried blaTEM gene which encodes an enzyme belongs to extendedspectrum β-lactamases (ESBLs), conferring significant penicillin resistance. MRSA strains which carried type II SCCmec element presenting characteristic of multidrug resistance (MDR), these strains harbored resistance genes ant(4’)-Ia and ermA, which showed positive correlation with kanamycin and erythromycin resistance, respectively. Genes aac(6’)-aph(2”) and tetM were also found in these strains which phenotypically associated with gentamicin and tetracycline resistance. In addition, type IVA was the most prevalent SCCmec element in our study, which carried a variant class B mec complex. The J3 region of type IVA element may integrate a copy of plasmid pUB110, harboring ant(4’)-Ia which phenotypically associated with kanamycin resistance. The molecular characteristics of MRSA strains in our study are unique compared with isolates which have spread internationally. This is the report to show the presence of blaTEM in meats isolated bacterias.

Biography

Hwang has completed his PhD from Inha University. He is the director of RIS, a Bio Health solution. He has published more than 50 papers in reputed journals.

E-mail: gene@gachon.ac.kr