Journal of Obesity & Eating Disorders

Influence of L-carnitine on the expression level of adipose tissue miRNAs related to weight changes in obese rats

7^th European Congress on Obesity and Eating Disorder
April 12-13, 2018 Amsterdam, Netherlands

Maryam Nazari, Majid Karandish and Alihossein Saberi

Ahvaz Jundishapur University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Obes Eat Disord

DOI: 10.21767/2471-8203-C1-009

Abstract

Background: Molecular mechanisms of most anti-obesity drugs are remained to be clear. MicroRNAs that are noncoding RNA molecules supposed to regulate biological processes concomitant to obesity and have attracted a lot of attention in scientific communities. miR-27a and miR143 expression levels in obese and non-obese rats during weight changes and L-carnitine (LC) effects on them was investigated in this study. Material & Methods: In the present study, 12 male Wistar rats were randomly divided into normal-fat diet (NFD) and high-fat diet (HFD) groups to develop obesity. After 8 weeks, rats were weighted and half of diet induced obese rats were randomly selected to receive 200 mg LC kg-1 body weight for 4 weeks. At the end, epididymal fat was isolated to investigate expression level of microRNAs by real-time PCR. Results: After 12 weeks, HFD in comparison with NFD caused significant decrease and increase in expression levels of miR27a and miR-143 respectively. These changes were modified in groups which had received LC in a 4-weeks period. Furthermore, rats in this group gained less weight. Main Conclusions: Findings of this study suggest that the changes of microRNAs expression probably play a role in pathogenesis of obesity. They might be modulated by means of dietary agents and supplements and modify weight gain trend.

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