Asian Journal of Plant Science & Research

Heterologous expression of a barley homologue Na+/K+ transporter from Leptochloa fusca showed ion permeability and salt tolerance

5^th Edition of International Conference on Plant Genomics
June 13-14, 2019 Berlin, Germany

Zaheer Ahmed

University of Agriculture, Pakistan

Posters & Accepted Abstracts: AJPSKY

Abstract

The aminoglycoside antibiotic hygromycin (hyg), inhibits yeast growth by hyperpolarization of cell membrane. Wild type and trk1, trk2 yeast (Saccharomyces cervisceae) strains growing on solid Yeast Peptone Dextrose (YPD) media showed growth sensitivity when supplemented with hyg. Growth of potassium uptake deficient yeast strain deprived of its two major K+ transporters; Trk1 and Trk2, was used to show the K+ uptake activities on expression of LfHKT2;1. Loss of growth activity of trk1, trk2 yeast was observed on expression of empty control vector in the presence of hyg while expression of LfHKT2;1 complemented the growth activity of trk1, trk2 yeast cells. Increased concentration of Na+ resulted in cellular Na+ toxicity which elevated on supplementing hyg in the media. Hypersensitivity of trk1, trk2 yeast cells to Na+ on expression of LfHKT2;1, clearly showed an additional Na+ uptake system operating on membrane as confirmed by transient GFP expression studies. Relative abundance of transcripts under low K+ concentration revealed the high affinity K+ uptake system on expression of LfHKT2;1. This study demonstrated the hyg sensitivity of yeast cells as the most powerful screen to functionally characterize membrane transporters and channels.

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