Nano Research & Applications

Evaluation of hemostatic behavior of gelatin-silica nanohybrid by changing the pH of injury in severe bleeding

17^th Edition of International Conference on Emerging Trends in Materials Science and Nanotechnology
April 26-27, 2018 Rome, Italy

S Moradi, M Chenani and S A Ahmaditabatabaei

Islamic Azad University, Tehran, Iran Imperial College London, UK

ScientificTracks Abstracts: Nano Res Appl

DOI: 10.21767/2471-9838-C1-008

Abstract

After trauma, fast hemostasis is an essential strategy in extensive bleeding, in this decade, much effort has been made to develop the hemostatic agents, but the existent options have ample restrictions, including failure to maintain the structure of the styptic in the face severe bleeding and rapid changes in pH. Since the changes in pH of injury site is an important factor in the failure of styptic and their structural damage, in this study gelatinsilica nanohybrid behavior in severe bleeding was evaluated under different pH. Experiments including blood absorption, zeta potential measurements, and poly disparity index by GPC tests were studied. By changing the pH of environment, structural integrity and there upon nanohybrid hemostatic behavior changed dramatically. So that nanohybrid showed the most blood absorption (440%) and acceded to a coherent structure with tendentious to alpha helix and beta-sheets (the secondary structure of a protein), that also provide ability to maintain integrity of structure in severe bleeding. These results obtained, in alkaline or acidic environment nanohybrid hemostatic behavior was limited, so that in the acidic pH, the blood absorption was reduced to 110% and 1.6 times the normal clotting time delayed. Based on the results of this study, it was found that changes in nanohybrid behavior in acidic pH were much more than in alkaline pH and nanohybrid can also maintain the structural integrity with rapid hemostasis. According to the desire of injury site to change the pH to alkaline side, the resulting nanohybrid has an ideal ability to control excessive bleeding and can be proposed for further studies in vivo as a novel styptic Recent Publications 1. Chenani M and Ahmadinejad M (2016) Preparation and characterization of novel gelatin/silica nanohybrid as a styptic for massive bleeding. Journal of Nanomaterials and Biostructures 4:1277- 1288. soodehmoradi@gmail.com