Journal of Transmitted Diseases and Immunity

Effect of laminin on neurotrophic factors expression in schwann-like cells induced from human adipose-derived stem cells in vitro

Joint Event on 22^nd Edition of International Conference on Immunology and Evolution of Infectious Diseases & 12^th Edition of International Conference on Tissue Engineering and Regenerative Medicine
May 10-11, 2018 Frankfurt, Germany

Mina Tadjalli, Giti Zarinfard and Shahnaz Razavi

School of Veterinary Medicine, Shiraz University, Iran School of Medicine, Isfahan University of Medical Sciences, Iran

Posters & Accepted Abstracts: J Transm Dis Immun

DOI: 10.21767/2573-0320-C2-006

Abstract

The Schwann-like cells can be considered as promising in stem cell therapies, at least in experimental models. Human adipose-derived stem cells (ADSCs) are induced into Schwannlike cells (SC-like cells) and are cultured on either a plastic surface or laminin-coated plates. The findings here reveal that laminin is a critical component in extracellular matrix (ECM) of SC-like cells at in vitro. The survival rate of SC-like cells on a laminin matrix are measured through MTT assay and it is found that this rate is significantly higher than that of the cells grown on a plastic surface (P < 0.05). Schwann cell markers and the myelinogenic ability of SC-like cells at the presence versus absence of laminin are assessed through immunocytochemistry. The analysis of GFAP/S100�?² and S100�?²/MBP markers indicate that laminin can increase the differentiated rate and myelinogenic potential of SC-like cells. The expression levels of SCs markers, myelin basic proteins (MBP), and neurotrophic factors in two conditions are analyzed by real-time reverse transcription polymerase chain reaction(RT-PCR).The findings here demonstrated that gene expression of SCs markers, MBP, and brain-derived neurotrophic factors (BDNF) increase significantly on laminin compared to plastic surface (P < 0.01). In contrast, the nerve growth factor(NGF) expression is down regulated significantly on laminin-coated plates (P < 0.05). The obtained data suggest that production of neurotrophic factors in SC-like cell in presence of laminin can induce appropriate microenvironment for nerve repair in neurodegenerative diseases.

Biography

Dr. Tadjalli is a professor of Histology and Zarinfard is a graduate student at the department of Histology  School of Veterinary Medicine, Shiraz University, Shiraz and Dr. Razavi  is a professor of Histology Isfahan University of Medical Science, Isfahan, Iran. Her research interest includes Histology, stem cell, regeneration.

Email:mtadjalli6@yahoo.com