Medical Mycology: Open Access

Distribution of amatoxins and phallotoxins in different tissues and development stages of Amanita subpallidorosea

2^nd International Conference on Mycology & Mushrooms
September 25-26, 2017 Chicago, USA

Shuo Zhang, Haijiao Li, Dawei Chen, Yunfeng Zhao and Chengye Sun

China National Center for Food Safety Risk Assessment, China
Chinese Center for Disease Control and Prevention, China

Posters & Accepted Abstracts: Med Mycol Open Access

DOI: 10.21767/2471-8521-C1-003

Abstract

Amanita subpallidorosea is a lethal mushroom discovered recently in China. Due to the morphological similarity to edible mushrooms and the high toxicity, A. subpallidorosea had caused severe mushroom poisonings in China. However, the contents and distribution of the major toxins in A. subpallidorosea remain poorly studied. In this study, the concentration of the major cyclopeptide toxins, amatoxins and phallotoxins in different tissues and development phases were systematically analyzed for the first time. To find other structure related high risk compounds which are not reported or available as standards to launch toxicological study in A. subpallidorosea, a new non-targeted strategy based on liquid chromatography-high resolution mass spectrometer (LCHRMS) was applied to analyze the toxin profiling of the mushroom and to find new cyclopeptide toxins. The results showed that the concentration of the total amatoxins in A. subpallidorosea were remarkably high, which was much higher than the worldly notorious A. phalloides, a lethal species from Europe and North America. The distribution of amatoxins and phallotoxins in different tissues showed the highest concentrations of amatoxins and phallotoxins were found in the cap and the lowest concentrations in the volva. Further analysis of mushrooms in different development stages showed that the amatoxin content was relatively high during early development, in which stage the fruit body grew most vigorously and regarded as tasty stage for mushroom picking. With the LCHRMS based strategy, seven cyclopeptides and two new compounds were found and confirmed by parallel reaction monitoring (PRM) and all ion fragmentation (AIF) mode on a high resolution hybrid quadrupole-orbitrap mass spectrometer.