Development of a novel adenovirus-based cancer immunotherapy that targets the SLAMF7 pathway and suppresses established tumors

Yasser Ali Aldhamen; Yuliya Pepelyayeva; Patrick O Connell; Sean Thomas Hyslop; Maja Kuranz Blake; Cristiane PereiraHicks; Sarah Godbehere Roosa; Andrea Amalfitano

doi:10.21767/2471-304X-C1-009

Development of a novel adenovirus-based cancer immunotherapy that targets the SLAMF7 pathway and suppresses established tumors

16^th EuroSciCon Conference on Immunology
March 11-12, 2019 | Amsterdam, Netherlands

Yasser Ali Aldhamen, Yuliya Pepelyayeva, Patrick O Connell, Sean Thomas Hyslop, Maja Kuranz Blake, Cristiane PereiraHicks, Sarah Godbehere Roosa and Andrea Amalfitano

College of Osteopathic Medicine-Michigan State University, USA

Posters & Accepted Abstracts: J Clin Immunol Allergy

DOI: 10.21767/2471-304X-C1-009

Abstract

Objective: HIV-1/AIDS therapy remains concerned problems due to side effects of highly active antiretroviral therapy. Thus, seeking of alternative strategies for HIV-1 inhibition is still needed. Houttuynia cordata Thunb (H. cordata) is a plant that possesses several antimicrobial activities. This study aimed to investigate the cytotoxicity of H. cordata extract on CD4+ lymphocytes; the anti-HIV-1 activity of the extract on CD4+ lymphocytes and the effects of the extract on anti-HIV-1 enzymatic activities.

Methods: Cytotoxicity of the extract was determined using MTT assay. To investigate anti-HIV-1 activity of H. cordata, C8166 cells were treated with the extract either before or after being exposed to HIV-1NPO3 and incubated for 72 h. The amounts of p24 antigen were detected by ELISA. Anti-HIV-1 integrase and protease activities of both the water and ethanolic extracts of H. cordata were determined using enzymatic assay.

Results: No cytotoxicity of H. cordata extract up to 400 μg/ml was observed. The extract showed potent anti-HIV-1 activity with 69% inhibition in pre-treated C8166 cells. However, HIV-1 replication was not significantly suppressed when the cells firstly exposed to the virus and then treated with the extract. H. cordata water extract possessed inhibitory effect against HIV-1 integrase while the ethanolic extract showed weakly inhibitory activity. Neither the water nor ethanolic extracts had anti-HIV-1 protease activity.

Conclusion: This study demonstrated that H. cordata can inhibit HIV-1 at the early stages of infection without cytotoxicity. These findings may lead to the development of a new effective anti-HIV-1 agent that is effective at the early stages of the infection.