Chronic Obstructive Pulmonary Disease: Open Access

Detection of JC Polyomavirus Tumor-Antigen In Gastric Carcinoma, a report from IRAN

CO-ORGANIZED EVENT:International Conference on Chronic Diseases & 6^th International Conference on Microbial Physiology and Genomics
August 31-September 01, 2017 Brussels, Belgium

Farnaz Zahedi Avval, Samira Izi, Farzad Rahmani, Nema Mohammadian Roshan, Atefeh Yari, Masoud Youssefi

Department of Clinical Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
Metabolic Syndrome Research Center, Nutrition and Biochemistry division, Mashhad University of Medical Sciences, Mashhad, IRAN
Department of Pathology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
Department of Microbiology and Virology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IRAN
Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, IRAN

Posters & Accepted Abstracts: Chron Obstruct Pulmon Dis

DOI: 10.21767/2572-5548-C1-003

Abstract

Background: Although the global incidence of gastric cancer has been declined dramatically over the recent few decades, it is still a common cancer in different parts of the world. It has been scientifically suggested that some oncogenic viruses like JCV might play a role in Gastric carcinogenesis. The viral transforming protein, T-antigen (T-Ag) has the ability to bind and inactivate tumor suppressor proteins including p53 and pRb that might lead to malignant outcome, though its role might vary in different geographic parts due to viral distribution and other habits resulting in GI cancer. Objective: The aim of present study was to investigate the presence of JCV T-Ag sequence and its expression in cancerous and non-cancerous adjacent gastric tissues in Iranian patients. Methods: Thirty one sample pairs of formalin fixed paraffin embedded (FFPE) tissue specimens of gastric cancer and adjacent non-cancerous tissues (ANCT) were investigated on the basis of Real-time polymerase chain reaction. Samples were subjected for the immunohistochemistry examination using an anti-T-Ag monoclonal antibody. Results: Real time experience followed by sequencing revealed JCV sequences in 17 (54.84%) of gastric cancer tissues and in 10 (32.25%) of non-cancerous gastric mucosa (OR=1.7). Immunohistochemical study also showed the presence of T-Ag in nuclear compartment. Conclusions: These data indicate, for the first time, presence of JC virus in gastric carcinoma samples in our socioeconomic region. These findings provide a summative, supportive data for a possible role of JCV T-Ag in carcinogenesis of gastric malignancy.