Nano Research & Applications

Design and manufacturing of nanobiosensors suitable for protein detection

EuroSciCon Conference on Nanotech & Nanobiotechnology Nano
July 12-13, 2018 Paris , France

Seyed Mohammad Mahdi Dadfar, Sylwia Sekula-Neuner, Uwe Bog, Vanessa Trouillet and Michael Hirtz

Karlsruhe Institute of Technology (KIT), Germany

ScientificTracks Abstracts: Nano Res Appl

DOI: 10.21767/2471-9838-C2-011

Abstract

Different types of click chemistry reactions have been proposed and used for the functionalization of surfaces and materials, and covalent attachment of organic molecules. In the present work, we present and compare two different catalyst-free click approaches, namely azide-alkyne and thiol-alkyne click chemistry, for the generation of nanobiosensors suitable for protein detection. For this purpose, we first functionalized the surface of glass with dibenzocyclooctyne-acid (DBCO-acid), a cyclooctyne with a carboxyl group. Then, the DBCO-terminated surfaces were functionalized with different fluorescent and nonfluorescent azide and thiol inks via microchannel cantilevers spotting (μCP) (Figure 1). Click reactions were performed at different temperatures and times and the optimum conditions of 37 °C/20 min and 37°C/40 min was found for azide-alkyne and thiol-alkyne reactions, respectively. Although, due to no need for catalysts or additional additives, mild reaction conditions, and high reaction rate, both routes worked reliable for surface functionalization, the protein binding experiments revealed that using a thiol-alkyne route will obtain the highest surface density of molecular immobilization in such spotting approaches. The obtained achievements and results from the protein binding experiments with streptavidin proved the potential for application of these microarrays in manufacturing nanobiosensors for protein detection and other biomedical/biological applications

Biography

Seyed Mohammad Mahdi Dadfar has completed his Bachelor and Master of Science at Tehran University and Shiraz University, respectively, both top-tier universities in Iran. He is pursuing his PhD at Karlsruhe Institute of Technology (KIT), Institute of Nanotechnology (INT) under supervision of PD Dr. Michael Hirtz and Prof. Dr. Annie Powell. His PhD project is about functionalized diamond optomechanical circuits for infrared spectroscopy and site-specific gas sensing applications. He is a Member of Iran’s National Elites Foundation (The highest prestige and professional nation foundation for supporting elites). Mahdi Dadfar has published more than 10 papers in reputed journals, holds two national patents and recently has submitted another paper during his PhD.

E-mail: mahdi.dadfar@kit.edu